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Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
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Product Citations
publications
EH12dot2H7_Pure_022508
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with purified CD279 (clone EH12.2H7) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
  • EH12dot2H7_Pure_022508
    PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with purified CD279 (clone EH12.2H7) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
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329902 100 µg 72€
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  2. Zheng G, et al. 2021. Signal Transduct Target Ther. 6:236. PubMed
  3. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  4. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  5. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  6. Gernoux G, et al. 2020. Mol Ther. 28:747. PubMed
  7. Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed
  8. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  9. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  10. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  11. Lu X, et al. 2018. Am J Cancer Res. 8:1564. PubMed
  12. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  13. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  14. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  15. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  16. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  17. Hsu DC, et al. 2018. Clin Infect Dis. 67:437. PubMed
  18. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  19. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  20. Mirzaei R, et al. 2021. Sci Adv. 7:eabh2148. PubMed
  21. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  22. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  23. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  24. Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
  25. Gee MH, et al. 2018. Cell. 172:549. PubMed
  26. Conrad J, et al. 2012. J Virol. 86:4213. PubMed
  27. Zahn R, et al. 2008. J Virol. 82:11577. PubMed
  28. Schwabenland M, et al. 2021. Immunity. . PubMed
  29. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  30. Stervbo U, et al. 2019. Front Immunol. 0.949305556. PubMed
  31. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  32. Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed
  33. Kim MH, et al. 2018. Cancer Immunol Res. 0.427083333. PubMed
  34. Conrad J, et al. 2011. J Immunol. 186:6871. PubMed
  35. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  36. Monteiro P, et al. 2011. J Immunol. 186:4618. PubMed
  37. Kim A, et al. 2019. J Immunol. 203:1076. PubMed
  38. Lewinsky H, et al. 2018. J Clin Invest. 128:5465. PubMed
  39. Vojnov L, et al. 2010. J Virol. 84:753. PubMed
  40. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  41. Nicholas K, et al. 2015. Cytometry A. 10.1002/cyto.a.22799. PubMed
  42. Hwang HJ, et al. 2021. Immun Inflamm Dis. 9:274. PubMed
  43. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  44. Enyindah-Asonye G, et al. 2019. Front Immunol. 10:511. PubMed
  45. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_940488 (BioLegend Cat. No. 329902)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Other Formats

View All CD279 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 421™ anti-human CD279 (PD-1) EH12.2H7 FC
Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC-F
FITC anti-human CD279 (PD-1) EH12.2H7 FC
PE anti-human CD279 (PD-1) EH12.2H7 FC,SB
APC anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 647 anti-human CD279 (PD-1) EH12.2H7 FC
PerCP/Cyanine5.5 anti-human CD279 (PD-1) EH12.2H7 FC
APC/Cyanine7 anti-human CD279 (PD-1) EH12.2H7 FC
Pacific Blue™ anti-human CD279 (PD-1) EH12.2H7 FC
PE/Cyanine7 anti-human CD279 (PD-1) EH12.2H7 FC
Purified anti-human CD279 (PD-1) (Maxpar® Ready) EH12.2H7 FC,CyTOF®
Brilliant Violet 605™ anti-human CD279 (PD-1) EH12.2H7 FC
Ultra-LEAF™ Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC-F
Brilliant Violet 711™ anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 785™ anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 510™ anti-human CD279 (PD-1) EH12.2H7 FC
Biotin anti-human CD279 (PD-1) EH12.2H7 FC
PE/Dazzle™ 594 anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 488 anti-human CD279 (PD-1) EH12.2H7 FC
PerCP anti-human CD279 (PD-1) EH12.2H7 FC
GoInVivo™ Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC
Brilliant Violet 650™ anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 700 anti-human CD279 (PD-1) EH12.2H7 FC
APC/Fire™ 750 anti-human CD279 (PD-1) EH12.2H7 FC
TotalSeq™-A0088 anti-human CD279 (PD-1) EH12.2H7 PG
TotalSeq™-B0088 anti-human CD279 (PD-1) EH12.2H7 PG
TotalSeq™-C0088 anti-human CD279 (PD-1) EH12.2H7 PG
Brilliant Violet 750™ anti-human CD279 (PD-1) EH12.2H7 FC
TotalSeq™-D0088 anti-human CD279 (PD-1) EH12.2H7 PG
PE/Fire™ 640 anti-human CD279 (PD-1) EH12.2H7 FC
PE/Cyanine5 anti-human CD279 (PD-1) EH12.2H7 FC
PE/Fire™ 744 anti-human CD279 (PD-1) EH12.2H7 FC
Spark Red™ 718 anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 570™ anti-human CD279 (PD-1) EH12.2H7 FC
Go To Top Version: 6    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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