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Purified anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_UCHT1_Purified_021805
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
  • 1_UCHT1_Purified_021805
    Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
  • 2_UCHT1_PURE_CD3_Antibody_IHC-F_090718
    Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD3 antibody (clone UCHT1, red) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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300401 25 µg 20€
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300402 100 µg 36€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP, Activ, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Law JC, et al. 2021. J Immunol. 206:37. PubMed
  3. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  4. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  5. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  6. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  7. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  8. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  9. Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  10. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  11. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  12. Pedersen JM, et al. 2023. Arthritis Res Ther. 25:97. PubMed
  13. Laurent A, et al. 2014. PLoS One. 9:103683. PubMed
  14. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  15. González-Mancha N, et al. 2022. Front Immunol. 12:814570. PubMed
  16. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  17. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  18. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  19. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  20. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  21. Kollis PM, et al. 2022. Front Immunol. 13:850226. PubMed
  22. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  23. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  24. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  25. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  26. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  27. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  28. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  29. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  30. Wang C, et al. 2022. Exp Ther Med. 23:221. PubMed
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  32. Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed
  33. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  34. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  35. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  36. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  37. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  38. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  39. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  40. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  41. Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
  42. Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed
  43. Khanolkar A, et al. 2020. Immunohorizons. 4:153. PubMed
  44. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  45. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  46. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  47. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  48. Jakob M, et al. 2020. Cells. 10:. PubMed
  49. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  50. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  51. Guerrero A, et al. 2012. J Immunol. 190:27. PubMed
  52. Wright CC, et al. 2017. Infect Immun. 85:e00131. PubMed
  53. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  54. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  55. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  56. Hartmann FJ, et al. 2019. Cell Rep. 28:819. PubMed
  57. Tang JS, et al. 2020. Food Funct. 11:5782. PubMed
  58. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  59. Schwabenland M, et al. 2021. Immunity. . PubMed
  60. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  61. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  62. Silva–Cardoso SC, et al. 2017. J Immunol. 199:253. PubMed
  63. Manes TL, et al. 2020. Free Radic Biol Med. 147:102. PubMed
  64. Thompson A, et al. 2004. J Immunol. 173:1671. PubMed
  65. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  66. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  67. Han M, et al. 2022. Cell Mol Immunol. 19:805. PubMed
  68. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  69. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  70. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  71. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  72. Kim ML, et al. 2021. iScience. 24:103509. PubMed
  73. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  74. Martin E, et al. 2020. JCI Insight. :5. PubMed
  75. Li J, et al. 2015. Cancer Res. 75:508. PubMed
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  77. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314055 (BioLegend Cat. No. 300401)
AB_314055 (BioLegend Cat. No. 300402)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 3    Revision Date: 09/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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