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Purified anti-human CD77 Antibody

Pricing & Availability
Clone
5B5 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD77.5
Other Names
globotriaosylcermamide (Gb3), Burkitt's lymphoma associated antigen (BLA), cermadie trihexoside (CTH), and Pk blood group antigen
Isotype
Mouse IgM, κ
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Product Citations
publications
5B5_Purified_CD77_Antibody_020813
Daudi, human B Burkitt's lymphoma cell line, was stained with purified CD77 (clone 5B5) (filled histogram) or mouse IgM, κ isotype control (open histogram), followed by anti-mouse IgM PE.
  • 5B5_Purified_CD77_Antibody_020813
    Daudi, human B Burkitt's lymphoma cell line, was stained with purified CD77 (clone 5B5) (filled histogram) or mouse IgM, κ isotype control (open histogram), followed by anti-mouse IgM PE.
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357101 25 µg 52€
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357102 100 µg 104€
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Description

CD77 is also known as Gb3 glycosphingolipid. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli. It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for CD77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral blood mononuclear cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include:  Western blotting1 and immunohistochemical staining of frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Fyfe G, et al. 1987. J. Immunol. 139:2187. (WB, IHC)
Product Citations
  1. Terada K, et al. 2024. J Cell Physiol. e31174:239. PubMed
  2. Berdasco C, et al. 2020. Front Cell Infect Microbiol. 0.681944444. PubMed
RRID
AB_2561847 (BioLegend Cat. No. 357101)
AB_2561847 (BioLegend Cat. No. 357102)

Antigen Details

Structure
Glycosphingolipid
Distribution

Burkitt's lymphoma cells, a subset of germinal center B lymphocytes

Function
Involved in cell adhesion and signaling transduction by binding CD19
Ligand/Receptor
Receptor of Shiga Toxin and Verotoxin 1 (VT1) and CD19
Cell Type
B cells, Lymphocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tétaud C, 2003. J. Biol. Chem. 278:45200.
2. Maloney MD, 1994. J. Exp. Med. 1994 180:191.
3. Mangeney M, et al. 1991. Eur. J. Immunol. 21:1131.
4. Mangeney M, et al. 1993. Cancer Res. 53:5314.

Gene ID
NA
Go To Top Version: 4    Revision Date: 10/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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