- Clone
- W16064A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon regulatory factor 7 (IRF-7) protein
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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696402 | 100 µg | 221€ |
IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse IRF7 recombinant protein (127-487a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
-
- RRID
-
AB_2687056 (BioLegend Cat. No. 696402)
Antigen Details
- Structure
- 457 amino acids, predicted molecular weight of 51.2 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
- Distribution
-
Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus.
- Function
- Transcription factor that transactivates proinflammatory cytokines and type I interferon genes in response to virus infection.
- Interaction
- Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429. - Gene ID
- 54123 View all products for this Gene ID
- UniProt
- View information about IRF7 on UniProt.org
Other Formats
View All IRF7 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-IRF7 | W16064A | WB |
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Purified anti-IRF7
Total cell lysates (15µg protein) from non-treated and recom...
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