- Clone
- Poly19052 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin, type II cytoskeletal 1, CK-1, cytokeratin-1, 67 kD cytokeratin, hair alpha protein, type-II keratin Kb1, epidermolytic hyperkeratosis 1, krt1
- Isotype
- Rabbit Polyclonal
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png 1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from A549 and HEL (negative controls) and HaCaT (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-KRT1 Antibody, clone Poly19052, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted KRT1 expression data obtained from Human Protein Atlas. -
IHC staining of anti-Keratin 1 antibody (Poly19052) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining. -
Western blot of anti-Keratin 1 antibody (Poly19052). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 24 hours at 4°C, followed by incubation with a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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905203 | 25 µg | 100€ | ||||
905204 | 100 µg | 280€ |
Keratin 1 is a member of the keratin family of proteins. It is a type II cytokeratin specifically expressed in the spinous and granular layers of the epidermis along with family member Keratin 10. Mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Reported Reactivity
- Non-Human Primate
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- The antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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IHC-P - Quality tested
IHC-F, WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.25 - 1.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2734677 (BioLegend Cat. No. 905203)
AB_2616954 (BioLegend Cat. No. 905204)
Antigen Details
- Structure
- Keratin 1 is a 66 kD protein.
- Distribution
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Extracellular, nucleus, and cytoskeleton.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 3848 View all products for this Gene ID
- UniProt
- View information about Keratin 1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Keratin 1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Keratin 1 | Poly19052 | IHC-P,IHC-F,WB |
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