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Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-14 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PD 1, PDCD1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP1-14_Pure_022309.jpg
Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
  • RMP1-14_Pure_022309.jpg
    Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
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114101 50 µg 67€
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114102 500 µg 102€
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze."
Application

FC - Quality tested
IHC, WB, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF™ purified antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
  2. Personal Communication (WB)
  3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
  4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
  5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
  7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
Product Citations
  1. Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed
  2. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  3. López-Medina M, et al. 2015. Immunobiology. . PubMed
  4. Wang G, et al. 2020. Nat Commun. 11:1395. PubMed
  5. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  6. Xu YY, et al. 2017. J Reprod Dev. 63:289. PubMed
  7. Tsang J, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Zhang H, et al. 2019. Mediators Inflamm. 2019:8492090. PubMed
  9. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  10. Jang SC, et al. 2021. Commun Biol. 4:497. PubMed
  11. Meng Q, et al. 2006. Invest Ophthalmol Vis Sci. 47:4444. PubMed
  12. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  13. Arana Y, et al. 2022. Front Immunol. 13:866120. PubMed
  14. Julian M, et al. 2015. PLoS One. 10: 0132921. PubMed
  15. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
RRID
AB_313572 (BioLegend Cat. No. 114101)
AB_313572 (BioLegend Cat. No. 114102)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 2    Revision Date: 02/17/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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