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Purified anti-mouse CD300LG (Nepmucin) Antibody

Pricing & Availability
Clone
ZAQ5 (See other available formats)
Regulatory Status
RUO
Other Names
CMRF35-like molecule 9, CLM-9, CD300g, D11Ertd736e
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
ZAQ5_Purified_CD300LG_Antibody_FC_012314
Mouse CD300LG-transfected L cells were stained with purified CD300LG (clone ZAQ5, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • ZAQ5_Purified_CD300LG_Antibody_FC_012314
    Mouse CD300LG-transfected L cells were stained with purified CD300LG (clone ZAQ5, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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147102 100 µg 226€
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Description

CD300LG, also known as nepmucin (a not expressed in Peyer's patches mucin), is a member of the CD300 antigen like family. It is a 50-95 kD glycoprotein with one IgV like domain and one mucin like domain and four isoforms are produced by alternative splicing. Nepmucin is expressed on high endothelial venules (HEV) from lymph nodes, but is absent on HEV from the Peyer's patches. CD300LG mediates lymphocyte adhesion through the intraction of its Ig domain with ICAM-1 and rolling on HEVs through the interaction of its mucin like domain with L-selectine. Also, it may play a role in the molecular transcytosis across the capillary endothelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Nepmucin-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
EM - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoelectron microscopy of peripheral LN HEVs1.

Application References

(PubMed link indicates BioLegend citation)
  1. Umemoto E, et al. 2006. J. Exp. Med. 203:1603. (EM)
  2. Jin S, et al. 2008. FEBS Lett. 582:3018.
  3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
  4. Umemoto E, et al. 2013. PLoS One 8:e83681.
RRID
AB_2562968 (BioLegend Cat. No. 147102)

Antigen Details

Structure
Sialomucin, member of the CD300 antigen like family, 50-95 kD. 1 IgV like domain, 1 mucin like domain. Four isoforms produced by alternative splicing.
Distribution

Expressed on high endothelial venules (HEVs) from lymph nodes but absent on HEVs from Peyer's patches, some myeloid cell lines.

Function
Mediator of lymphocyte adhesion and rolling on HEVs, possible role in transcytosis across the capillary endothelium.
Ligand/Receptor
L-selectin.
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Takatsu H, et al. 2006. Biochem. Biophys. Res. Commun. 348:183.
2. Umemoto E, et al. 2006. J. Exp. Med. 203:1603.
3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.

Gene ID
52685 View all products for this Gene ID
UniProt
View information about CD300LG on UniProt.org
Go To Top Version: 1    Revision Date: 01/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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