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Purified anti-mouse CD40 Antibody

Pricing & Availability
Clone
HM40-3 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Armenian Hamster IgM
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Product Citations
publications
HM40-3_PURE_CD40_Antibody_120919_updated
C57BL/6 mouse splenocytes stained with CD3ε APC and CD40 (clone HM40-3) purified (left) or Armenian Hamster IgM isotype control (right) followed by Biotin anti-Armenian Hamster IgG and Streptavidin-PE.
  • HM40-3_PURE_CD40_Antibody_120919_updated
    C57BL/6 mouse splenocytes stained with CD3ε APC and CD40 (clone HM40-3) purified (left) or Armenian Hamster IgM isotype control (right) followed by Biotin anti-Armenian Hamster IgG and Streptavidin-PE.
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102901 50 µg 57€
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102902 500 µg 184€
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Description

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
(BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, Costim, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102908).

Application References

(PubMed link indicates BioLegend citation)
  1. Kawano T, et al. 1997. Science 278:1626. (Block)
  2. Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
  3. Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
  4. Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
  5. Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
  6. Ridge JP, et al. 1998. Nature 393:474. (Costim)
  7. Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
  8. Ma XT, et al. 2006. Cancer Research 66:1169.
  9. Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  3. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  4. Lindner SE, et al. 2020. Immunohorizons. 0.356944444. PubMed
  5. Yu X, et al. 2006. J Biol Chem. 281:15505. PubMed
  6. Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed
  7. Michaud D, et al. 2020. Cytokine. 125:154817. PubMed
  8. Sundaravinayagam D et al. 2019. Cell Rep. 28(6):1389-1399 . PubMed
  9. Balasubramanian S, et al. 2022. Elife. 11:. PubMed
  10. Garg G, et al. 2017. Oncotarget. 8:35542. PubMed
  11. Ait-Oufella H, et al. 2010. J Exp Med. 207:1759. PubMed
  12. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
RRID
AB_312945 (BioLegend Cat. No. 102901)
AB_312945 (BioLegend Cat. No. 102902)

Antigen Details

Structure
TNFR superfamily, 48 kD
Distribution

B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors

Function
Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor
CD40L (CD154)
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 2    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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