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Purified anti-mouse CLEC-2 (CLEC1B) Antibody

Pricing & Availability
Clone
17D9/CLEC-2 (See other available formats)
Regulatory Status
RUO
Other Names
CLEC1B, CLEC1B1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
17D9_Purified_CLEC-2_Antibody_FC_062613
C57BL/6 mouse platelets were stained with purified CLEC-2 (clone 17D9/CLEC-2) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • 17D9_Purified_CLEC-2_Antibody_FC_062613
    C57BL/6 mouse platelets were stained with purified CLEC-2 (clone 17D9/CLEC-2) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG PE.
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146102 100 µg 188€
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Description

CLEC-2, also known as CLEC1B and CLEC1B1, is C-type lectin-like receptor with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin; podoplanin has also been identified as an endogenous ligand for CLEC-2. Exposure of platelets to rhodocytin leads tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. CLEC-2 also functions as an activation receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Mourpo-Sß D, et al. 2011. Eur. J. Immunol. 41:3040. (WB)
Product Citations
  1. Bergsma A, et al. 2019. Bone Rep. 10:100196. PubMed
RRID
AB_2562240 (BioLegend Cat. No. 146102)

Antigen Details

Structure
Type II transmembrane protein with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM)
Distribution

Platelets, megakaryocytes, granulocytes, monocytes, macrophages, and dendritic cells

Function
Involved in platelet aggregation and secretion as well as neutrophil phagocytosis
Ligand/Receptor
Venom toxin, rhodocytin, and podoplanin
Cell Type
Dendritic cells, Granulocytes, Macrophages, Megakaryocytes, Monocytes, Platelets, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Boulaftali Y, et al. 2013. J. Clin. Invest. 123:908. 
2. Manne BK, et al. 2013. J. Biol. Chem. 288:7717.
3. Acton SE, et al. 2012. Immunity. 37:276. 
4. Finney BA, et al. 2012. Blood 119:1747. 
5. Kerrigan AM, et al. 2009. J. Immunol. 182:4150.

Gene ID
56760 View all products for this Gene ID
UniProt
View information about CLEC-2 on UniProt.org
Go To Top Version: 2    Revision Date: 10/13/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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