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Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
H22 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
H22_PURE_IFNgamma_Antibody_WB_050416
Western blot analysis of 50 ng recombinant human IFN-γ (carrier-free) (lane 1), recombinant rat IFN-γ (carrier-free) (lane 2), and recombinant mouse IFN-γ (carrier-free) (lane 3) using anti-mouse IFN-γ (clone H22) antibody followed by HRP goat anti-Armenian hamster IgG secondary antibody.
  • H22_PURE_IFNgamma_Antibody_WB_050416
    Western blot analysis of 50 ng recombinant human IFN-γ (carrier-free) (lane 1), recombinant rat IFN-γ (carrier-free) (lane 2), and recombinant mouse IFN-γ (carrier-free) (lane 3) using anti-mouse IFN-γ (clone H22) antibody followed by HRP goat anti-Armenian hamster IgG secondary antibody.
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513202 100 µg 81€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Recombinant full-length murine IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schreiber RD, et al. 1985. J. Immunol. 134:1609.
  2. Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
  3. Steed AL, et al. 2006. J. Virol. 80:192.
  4. Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed
Product Citations
  1. Wang L, et al. 2022. Cell Rep. 41:111462. PubMed
  2. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  3. Chang Q, et al. 2020. Scand J Immunol. 92:e12877. PubMed
  4. Wu L, et al. 2014. Am J Pathol. 184:2260. PubMed
RRID
AB_1089144 (BioLegend Cat. No. 513202)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 5    Revision Date: 01/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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