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Purified anti-mouse IL-25 (IL-17E) Antibody

Pricing & Availability
Clone
M3706F07 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 25, Interleukin 17E
Isotype
Rat IgG2a, κ
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Product Citations
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  • M3706F07_Purified_IL-25_Antibody_070119.png
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676304 500 µg 465€
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Description

IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49%), while mouse IL-17A and IL-25 have the lowest (28%). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses; this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse IL-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1 - 8 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-25 (IL-17E) recombinant protein ranging from 1600 to 25 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The pure M3706F07 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M3704D11 antibody (Cat. No. 538104) as the detection antibody and Recombinant Recombinant Mouse IL-25 (IL-17E) (ELISA Std.) (Cat. No. 579809).

RRID
AB_2814536 (BioLegend Cat. No. 676304)

Antigen Details

Structure
Heterodimer
Distribution

IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

Function
IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
Interaction
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
Ligand/Receptor
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD, et al. 2002. J. Immunol. 169:443.
  2. Ikeda K, et al. 2003. Blood 101:3594.
  3. Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
  4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
  5. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
  6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250
Gene ID
140806 View all products for this Gene ID
UniProt
View information about IL-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-mouse IL-25 (IL-17E)

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