Purified anti-mouse LIF Antibody

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Clone: Poly5232 (See other available formats)
Regulatory Status: RUO
Other Names: Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
Isotype: Goat Polyclonal IgG
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523201

100 µg

280€

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Description

Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant Mouse LIF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of LIF recombinant protein ranging from 1200 to 18.75 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The biotinylated Poly5232 (Cat. No. 523204) antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure Poly5232 antibody (Cat. No. 523201) as the capture antibody and Recombinant Recombinant Mouse LIF (ELISA Std.) (Cat. No. 554009).
RRID: AB_2814445 (BioLegend Cat. No. 523201)

Antigen Details

Structure: Monomer
Distribution: Expressed in the trophectoderm of the developing embryo, activated CD4⁺ T cells
Function: Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
Interaction: Embryonic stem cells
Ligand/Receptor: LIFR
Gene ID: 16878 View all products for this Gene ID
UniProt: View information about LIF on UniProt.org

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Other Formats

View All LIF Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse LIF	Poly5232	ELISA Capture
Biotin anti-mouse LIF	Poly5232	ELISA Detection
LEAF™ Purified anti-mouse LIF	Poly5232	Neut,WB

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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