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Purified anti-phycoerythrin (PE) Antibody

Pricing & Availability
Clone
PE001 (See other available formats)
Regulatory Status
RUO
Other Names
PE
Isotype
Mouse IgG1, κ
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Product Citations
publications
PE001_Pure_042909.jpg
C57BL/6 splenocytes stained with RM4-5 PE and purified PE001, then detected with anti-mouse IgG1 FITC
  • PE001_Pure_042909.jpg
    C57BL/6 splenocytes stained with RM4-5 PE and purified PE001, then detected with anti-mouse IgG1 FITC
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Cat # Size Price Quantity Check Availability Save
408101 50 µg 67€
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408102 500 µg 235€
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Description

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cyanine5, PE/Cyanine5.5 and PE/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phycoerythrin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Campisi L, et al. 2022. Nature. 606:945. PubMed
  3. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
  4. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  5. Dolton G, et al. 2022. Cell. 185:2936. PubMed
  6. Kwon M, et al. 2020. Clin Cancer Res. 1644:26. PubMed
  7. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  8. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  9. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  10. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  11. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  12. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  13. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  14. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  15. Rius C, et al. 2018. J Immunol. 200:2263. PubMed
  16. Smith CC, et al. 2019. Cancer Immunol Res. 1.396527778. PubMed
  17. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
RRID
AB_2168924 (BioLegend Cat. No. 408101)
AB_2168924 (BioLegend Cat. No. 408102)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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