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Purified anti-PLK-1 Phospho (Thr210) Antibody

Pricing & Availability
Clone
2A3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2A3_PURE_PLK1-Phospho-Thr210_Antibody_1_120619
Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-PLK-1 Phospho (Thr210) Antibody, clone 2A3, 2hrs at RT. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti PLK-1 Antibody (Cat. No. 627702) was used as a pan PLK-1 loading control. Lane M: Molecular weight marker.
  • 2A3_PURE_PLK1-Phospho-Thr210_Antibody_1_120619
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-PLK-1 Phospho (Thr210) Antibody, clone 2A3, 2hrs at RT. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti PLK-1 Antibody (Cat. No. 627702) was used as a pan PLK-1 loading control. Lane M: Molecular weight marker.
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628901 25 µg 95€
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628902 100 µg 220€
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Description

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Molli PR, et al. 2010. J Cell Biol. 190:101. PubMed
  2. Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
Product Citations
  1. Pop OT, et al. 2023. Cell Mol Gastroenterol Hepatol. 17:16. PubMed
  2. Bucko PJ, et al. 2019. eLife. 0.333333333333333. PubMed
  3. Bucko PJ, et al. 2020. J Biol Chem. 295:13784. PubMed
  4. Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
  5. Molli P, et al. 2010. J Cell Biol. 190:101. PubMed
  6. Tankiewicz‐Kwedlo A et al. 2017. British journal of pharmacology. 175(5):743-762 . PubMed
RRID
AB_439786 (BioLegend Cat. No. 628901)
AB_439786 (BioLegend Cat. No. 628902)

Antigen Details

Structure
Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution

Nuclear protein, highly expressed in placenta and colon

Function
Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Interaction
Interacts with nuclear distribution gene C
Modification
Phosphorylation (Thr210 is major phosphorylation site in activated PLK-1 from mitotic cells)
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.

Regulation
Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Gene ID
5347 View all products for this Gene ID
UniProt
View information about PLK-1 Phospho Thr210 on UniProt.org
Go To Top Version: 4    Revision Date: 12/06/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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