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- Clone
- 341 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD8, Lyt3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 200702 | 500 µg | 141€ | ||||
CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD8+ Wistar rat splenic T cell hybridomas
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, WB, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.
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Application References
(PubMed link indicates BioLegend citation) -
- Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB)
- Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC)
- Hirji N, et al. 1997. J. Immunol. 158:1833.
- Hirji N, et al. 1998. J. Immunol. 160:6004.
- Product Citations
-
- RRID
-
AB_313883 (BioLegend Cat. No. 200702)
Antigen Details
- Structure
- Ig superfamily, CD8β chain
- Distribution
-
Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages
- Function
- Co-receptor for TCR
- Ligand/Receptor
- MHC class I molecule
- Cell Type
- Macrophages, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
2. Kuhnlein P, et al. 1994. J. Immunol. 153:979.
3. Hirji N, et al. 1997. J. Immunol. 158:1833.
4. Hirji N, et al. 1998. J. Immunol. 160:6004. - Gene ID
- 24931 View all products for this Gene ID
- UniProt
- View information about CD8beta on UniProt.org
Related FAQs
Other Formats
View All CD8b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat CD8b | 341 | FC,IP,WB,IHC-F |
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Purified anti-rat CD8b
LOU rat splenocytes stained with purified 341, followed by a...
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