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Purified anti-SNAP-25 Antibody

Pricing & Availability
Clone
SMI 81 (See other available formats)
Regulatory Status
RUO
Other Names
Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-81_Pure_SNAP25_Antibody_1_081718
IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_1_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_2_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_3_081718
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-81_Pure_SNAP25_Antibody_4_081718
    Western blot of purified anti-SNAP-25 antibody (clone SMI 81). Lane 1: Molecular weight marker; Lane 2: 15 µg of human brain lysate; Lane 3: 15 µg of mouse brain lysate; Lane 4: 15 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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836303 25 µg 90€
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836304 100 µg 221€
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Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length, unaltered SNAP-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
EM, FC, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
  2. Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
  3. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
  4. Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
  5. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
  6. Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
  7. Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
  8. Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
Product Citations
  1. Junakovic A, et al. 2023. Brain Struct Funct. 228:613. PubMed
  2. Wang Q, et al. 2023. Front Mol Biosci. 10:1163545. PubMed
  3. Brinkmalm A, et al. 2014. Mol Neurodegener. 9:53. PubMed
  4. Hirano A, et al. 2011. J Comp Neurol. 519:972-988. PubMed
  5. Wang W, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Marcelli S, et al. 2019. Sci Rep. 9:7146. PubMed
  7. Gu Y, et al. 2016. Proc Natl Acad Sci U S A. 113: 922 - 931. PubMed
  8. Kandachar V, et al. 2018. J Cell Sci. 131:jcs222034. PubMed
  9. Xie YX, et al. 2022. Nat Commun. 13:4918. PubMed
  10. Konstantinović J, et al. 2018. J Med Chem. 61:1595. PubMed
  11. Stewart M, et al. 2019. Dis Model Mech. 12:. PubMed
  12. Wilhelmi I, et al. 2020. Mol Metab. 45:101151. PubMed
  13. Kabachinski G, et al. 2016. Mol Biol Cell. 27: 654 - 668. PubMed
  14. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  15. Kunii M, et al. 2016. J Cell Biol. 215: 121 - 138. PubMed
RRID
AB_2566521 (BioLegend Cat. No. 836303)
AB_2566521 (BioLegend Cat. No. 836304)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum
Cellular distribution: Cytosol, golgi apparatus, and cell membrane

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org
Go To Top Version: 3    Revision Date: 04/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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