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Purified anti-Syk Antibody

Pricing & Availability
Clone
4D10.2 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
a-4D10-2_Purified_Syk_Antibody_1_031919
Total cell lysates (15 µg total protein) from A549 (negative control), RPMI-8226, Daudi, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-Syk Antibody, clone 4D10.2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a-4D10-2_Purified_Syk_Antibody_1_031919
    Total cell lysates (15 µg total protein) from A549 (negative control), RPMI-8226, Daudi, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-Syk Antibody, clone 4D10.2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-4D10_WB_05-06-09
    Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Syk (clone 4D10.2) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • c-4D10-2_Purified_Syk_Antibody_3_072722
    IHC staining using purified anti-Syk (clone 4D10.2) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA, pH: 9.0, the tissue was incubated with (B) or without (A) 10 μg/mL of anti-Syk antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10x objective. Scalebar = 50 μM
  • d-4D10-2_Purified_Syk_Antibody_4_072722
    IHC staining using purified anti-Syk (clone 4D10.2) on formalin-fixed paraffin-embedded human tonsil tissue. The image was captured with a 40x objective. Scalebar = 50 μM
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644301 25 µg 95€
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644302 100 µg 235€
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Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Syk peptide aa314-339
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
IP, ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg per ml antibody dilution buffer. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Van Oers, et al. 1995. J. Exp. Med. 182:1585
  2. Chu DH, et al. 1999. J. Immunol. 163:2610.
Product Citations
  1. Bauer B, et al. 2017. J Biol Chem. 292(8):3213-3223. PubMed
  2. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
RRID
AB_2287062 (BioLegend Cat. No. 644301)
AB_2287062 (BioLegend Cat. No. 644302)

Antigen Details

Structure
Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution

Cytoplasmic protein, widely expressed in hematopoietic cells

Function
Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.

Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk on UniProt.org

Other Formats

View All Syk Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Syk 4D10.2 WB,IP,ICFC,IHC-P
PE anti-Syk 4D10.2 ICFC
APC anti-Syk 4D10.2 ICFC
Go To Top Version: 3    Revision Date: 07/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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