Loading...

Purified anti-Syntrophin Antibody

Pricing & Availability
Clone
1351 (See other available formats)
Regulatory Status
RUO
Other Names
Dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNT1, LQT12,dJ1187J4.5
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1351_PURE_Syntrophin_Antibody_WB_090915
Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
  • 1351_PURE_Syntrophin_Antibody_WB_090915
    Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
Cat # Size Price Quantity Check Availability Save
845102 100 µg 268€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Syntrophins are a family of five 60 kD adapter proteins that use their multiple protein interaction domains (two PHDs and a PDZ) to localize various signaling proteins (kinases, ion/water channels, or NOS) to specific intracellular locations. In neurons, syntrophins are known as the postsynaptic density protein (PSD) that serves to connect neurotransmitter receptors (such as nAChRs) to the cytoskeleton.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope has been mapped to amino acids 108-120 within the a-syntrophin PDZ domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC)
  2. Gee S, et al. 1998. J. Neurosci. 18:128. (IP)
  3. Ort T, et al. 2001. EMBO 20:4013. (IP, WB)
  4. Adams M, et al. 2001. J. Cell Biol. 155:113. (IF)
  5. Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
Product Citations
  1. Martin CA, et al. 2022. Regen Biomater. 9:rbac031. PubMed
RRID
AB_2566133 (BioLegend Cat. No. 845102)

Antigen Details

Structure
Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
Function
Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
Interaction
Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
6640 View all products for this Gene ID
UniProt
View information about Syntrophin on UniProt.org
Go To Top Version: 1    Revision Date: 09/17/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account