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Purified anti-Wntless (GPR177) Antibody

Pricing & Availability
Clone
YJ5 (See other available formats)
Regulatory Status
RUO
Other Names
G Protein-Coupled Receptor 177 (GPR177), WLS, Protein Wntless Homolog, Putative NF-Kappa-B-Activating Protein 373, C1orf139, Evi
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
YJ5_Purified_Wntless_Antibody_2_042419.png
Whole cell extracts (15 µg protein) from HepG2 (negative control) or HaCaT cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Wntless (GPR177) Antibody, clone YJ5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • YJ5_Purified_Wntless_Antibody_2_042419.png
    Whole cell extracts (15 µg protein) from HepG2 (negative control) or HaCaT cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Wntless (GPR177) Antibody, clone YJ5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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655902 100 µg 278€
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Description

Wnt signaling plays important roles in embryonic development and cancer formation. Wntless (GPR177) is a putative G-protein coupled receptor that regulates Wnt's trafficking and secretion. It can regulate the expression, subcellular location, binding, and organelle-specific association of Wnt proteins. In mammals, Wntless is mainly expressed in submucosa, muscle, ganglia, and nerve cells of the large intestines. Overexpression of Wntless may activate the NF-κB signaling pathway in cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Wntless protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone recognizes a ~65 kD protein of unidentified origin.

Additional reported applications (for relevant formats) include: immunoprecipitation (IP)1 and immunocytochemistry (ICC)1

Application References

(PubMed link indicates BioLegend citation)
  1. Coombs GS, et al. 2010. J. Cell Sci. 123:3357. (ICC, IP)
Product Citations
  1. Wesslowski J, et al. 2020. J Biol Chem. 295:8759. PubMed
  2. Glaeser K, et al. 2018. EMBO J. 37:. PubMed
RRID
AB_2562228 (BioLegend Cat. No. 655902)

Antigen Details

Structure
Three isoforms with 450 to 543 amino acids, predicted molecular weight of 45-65 kD; structure similar to the member of G-protein coupled receptor (GPCR) superfamily, with conserved YXXΦ endocytotic motif
Distribution

Cytoplasmic organelles

Function
Regulates Wnt protein sorting and secretion; it plays a key role in the regulation of expression, subcellular localization, binding and organelle-specific association of Wnt proteins
Interaction
Interacts with WNT1, WNT3, WNT5A, and Vps35
Ligand/Receptor
Wnt
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Signal Transduction
Antigen References

1. Hardin J and King RS, et al. 2008. Curr. Opin. Genet. Dev. 18:362.
2. Wang LT, et al. 2012. Kaohsiung J. Med. Sci. 28:355.
3. Cornett B, et al. 2013. Dev. Biol. 2013. 379:38.
4. Das S, et al. 2012. Front. Biol. (Beijing). 7:587.
5. Stefater JA 3rd, et al. 2013. Blood 121:2574.

Gene ID
79971 View all products for this Gene ID
UniProt
View information about Wntless on UniProt.org
Go To Top Version: 4    Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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