Recombinant Human FGF-8a (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Fibroblast growth factor 8, FGF8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8
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Product Citations
publications
Human_FGF-8a_CF_RECOM_1_051018
Recombinant human FGF-8a induces proliferation of BALB/c 3T3 cell in a dose dependent manner with ED50 of 0.1 - 0.4 µg/mL. The Deep Blue Cell Viability™ Kit (Cat. No. 424702) was used measure the number of cells.
  • Human_FGF-8a_CF_RECOM_1_051018
    Recombinant human FGF-8a induces proliferation of BALB/c 3T3 cell in a dose dependent manner with ED50 of 0.1 - 0.4 µg/mL. The Deep Blue Cell Viability™ Kit (Cat. No. 424702) was used measure the number of cells.
  • Human_FGF-8a_CF_RECOM_2_051018
    Stability testing for human FGF-8a. Human FGF8-8a was aliquoted in 20 mM Tris, 150 mM Na2SO4, 1 mM EDTA, pH 8, 10% glycerol at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in BALB/c 3T3 cell proliferation assay.
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773508 500 µg 2109€
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773502 10 µg 118€
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773504 25 µg 165€
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773506 100 µg 676€
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Description

FGF-8 belongs to the fibroblast growth factor (FGF) family and plays an important role in cell growth, embryogenesis, and tumorigenesis. Four isoforms of FGF-8 in human (a, b, e, f) and eight isoforms in mouse (a-h) generated by alternative splicing of the mRNA have been described. Comparing human and mouse, FGF-8a and FGF-8b show identical sequence homology. FGF-8b is predominant form and co-expressed with FGF-8a. The isoforms have different biological functions during embryogenesis. During prenatal stages, FGF-8 is required for the normal development of various organs, including limbs and central nervous system. Misexpression of FGF-8a and 8b proteins results in dysregulation of fate determination in developing brain. In addition, FGF-8 was first cloned from SC-3 mouse mammary carcinoma cells and was found to be induced in response to androgenic stimuli. The isoform FGF-8b has the highest affinity to FGF receptors, elucidating the stronger transforming capacity than FGF-8a. FGF-8b is detected in human prostate and breast carcinoma specimens and cell lines. FGF-8f is associated with the prognosis of esophageal carcinomas. FGF-8e mutation is associated with deficiency of gonadotropin-releasing hormone (GnRH).

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human FGF-8a, amino acids Gln23 - Arg204 (Accession #: NP_149355), with an N-terminal Met, was expressed in E. coli.
Molecular Mass
The 183 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kD. The DTT-reduced and non-reduced protein migrates at approximately 22 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Formulation
0.22 µm filtered protein solution is in 20 mM Tris, 150 mM Na2SO4, 1 mM EDTA, pH 8, 10% glycerol.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Preparation
> 98%, as determined by Coomassie stained SDS-PAGE.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 0.1 - 0.4 µg/mL, as determined by a dose-dependent stimulation of BALB/c 3T3 cell proliferation in the presence of 10 µg/mL heparin.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Falardeau J, et al. 2008. J. Clin. Invest. 118:2822.
  2. Turner N, Grose R. 2010. Nat. Rev. Cancer. 10:116.
  3. Tanaka S, et al. 2001. Dig. Dis. Sci. 46:1016.
  4. Payson RA, et al. 1996. Oncogene. 13:47.
  5. Gemel J, et al. 1996. Genomics. 35:253.
  6. Han MJ, et al. 2001. Dev Dyn. 220:40.
  7. Sunmonu NA, et al. 2011. J. Cell. Physiol. 226:1722.

Antigen Details

Structure
Growth factor
Distribution

Secreted protein; widely expressed during embryonic stages, such as the primitive streak, limb bud and midbrain-hindbrain junction; low expression levels in adult hematopoietic cells and gonads.

Function
FGF-8 is involved in the regulation of cell proliferation and cell differentiation during embryonic development.
Ligand/Receptor
FGFR1, FGFR2, FGFR3 and FGFR4.
Bioactivity
Measured by its ability to induce proliferation of BALB/c 3T3 cells.
Cell Targets
Embryonic progenitor cells.
Cell Type
Embryonic Stem Cells
Biology Area
Stem Cells
Gene ID
2253 View all products for this Gene ID
UniProt
View information about FGF-8a on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 05/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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