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- Regulatory Status
- RUO
- Other Names
- Interleukin-22 Receptor Subunit Alpha-2, IL-22 receptor-alpha 2, IL-22R-alpha-2, IL-22RA2, Interleukin 22-Binding Protein, 22 Receptor Subunit Alpha-2, IL-22BP, Cytokine Receptor Family 2 Member 10, IL-22 Receptor Subunit Alpha-2
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- Product Citations
- publications
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Human IL-22BP inhibits L-10 production induced by IL-22 in Colo 205 human colon adenocarcinoma cells.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 766508 | 500 µg | 1688€ | ||||
| 766502 | 10 µg | 118€ | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-22BP is a class 2 cytokine receptor family member (CRF2); it is a soluble receptor that does not have transmembrane and cytosolic domains. IL-22BP binds to IL-22 and does not bind to other members of the IL-10 family. IL-22 signals through the mechanism that involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain. IL-22R1 expression is restricted to epithelial cells, hepatocytes, and acinar cells of the pancreas, whereas IL-10Rβ1 expression is ubiquitous. IL-22BP prevents the binding of IL-22 to the membrane bound IL-22R1. IL-22 has high affinity binding for IL-22BP, and it is 20- to 1000-fold higher compared to its binding to the membrane bound IL-22R1. IL-22BP inhibits IL-22 biological effects in vitroand in vivoand is constitutively expressed in secondary lymphoid organs, breast, and epithelial tissues such as gut, lung, and skin.IL-22BP plays a key role in controlling tumorigenesis and epithelial cell proliferation in the colon. High expression of IL-22BP has been detected in gastric adenoma tissues. In addition, non-affected skin of psoriasis patients shows lower expression of IL-22BP than skin of healthy controls; this decrease in IL-22BP exacerbates psoriatic skin inflammation.
Product DetailsProduct Details
- Source
- Human IL-22BP, amino acids (Thr23-Pro231) (Accession # NM_181309) was expressed in 293E. The carboxy terminus contais hIgG Fc tag.
- Molecular Mass
- The 449 amino acid recombinant protein has a predicted molecular mass of approximately 51.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Thr.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human IL-22BP inhibits the production of IL-10 induced by IL-22 (BL Cat# 571302) in Colo 205 human colon adenocarcinoma cells in a dose dependent manner. The ED50 = 3 - 15 ng/ml.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer (soluble receptor)
- Distribution
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Activated B cell, monocytes, dendritic cells, and epithelial cells. It is induced by retinoic acid in immature monocyte-derived DC, and it is downregulated in mature DC.
- Function
- Antagonize the activity of IL-22, regulation of inflammatory responses. It is down regulated by IL-18 in intestinal tissue damage.
- Ligand/Receptor
- IL-22 (IL-10-related T cell-derived inducible factor, IL-TIF)
- Bioactivity
- IL-22BP inhibits the production of IL-10 induced by IL-22 in Colo 205 human cells.
- Biology Area
- Immunology
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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1. Dumoutier L, et al. 2001, J. Immunol. 166:7090.
2. Xu W, et al. 2001, Proc. Natl. Acad. Sci. U S A. 98:9511.
3. Logsdon NJ, et al. 2002, J. Interferon Cytokine Res. 22:1099.
4. Bleicher L, et al. 2008, FEBS Lett. 582:2985.
5. Zheng Y, et al. 2008, Nat. Med. 14:282.
6. Huber S, et al. 2008, Nature 491:259.
7. Martin JC, et al. 2014, Mucosal Immunol. 7:101.
8. Yang SB, et al. 2016, Oncol. Lett. 12:1477.
9. Martin JC, et al. 2017, J. Immunol. 198:3671. - Gene ID
- 116379 View all products for this Gene ID
- UniProt
- View information about IL-22Ra2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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