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Retrieve-All Antigen Unmasking #1: Universal 100X (Previously Covance catalog# SIG-31910)

Pricing & Availability
Regulatory Status
RUO
Other Names
Retrieve-All Antigen Unmasking Solution
Previously
Signet Catalog# 1910
Covance Catalog# SIG-31910
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927901 50 mL 272€
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Technical Data Sheet (pdf)

Product Details

Formulation
Antigen Unmasking Solution.
Storage & Handling
Store at room temperature.
Application

IHC
Recommended Usage

The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator.

The Retreive-All working solution should be prepared by mixing 10 mL of 100x Retrieve-All into 990mL of water.

Protocol
1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 min.
2. Preheat Retrieve-All buffer to 92°C*.
3. Place slides into the preheated Retrieve-All buffer.
4. Incubate for 10 minutes at 92°C.
5. Remove the container with slides and let it cool at room temperature for 5-10 minutes.
6. Rinse in distilled water or PBS.
7. Proceed with usual immunohistochemical staining.

Application Notes

*Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to the HIER.

Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures:

Retrieve-All 1 (Universal pH 8)
Retrieve-All 2 (Basic pH 10)
Retrieve-All 3 (Acidic pH 4.8) 

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 03/27/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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