Spark NIR™ 685 anti-human CD69 Antibody, CD69, FN50

Pricing & Availability

Clone: FN50 (See other available formats)
Regulatory Status: RUO
Workshop: IV A91
Other Names: Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype: Mouse IgG1, κ
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Product Citations: publications

FN50_SparkNIR685_CD69_Antibody_092520 — Human peripheral blood lymphocytes were stimulated with PMA+ ionomycin for 6 hours and then stained with CD69 (clone FN50) Spark NIR™ 685 (filled histogram) or mouse IgG1 κ, Spark NIR™ 685 isotype control (open histogram).

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310957

25 tests

100€

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310958

100 tests

235€

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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4⁺ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections², immunofluorescence microscopy³, and spatial biology (IBEX)^8,9.
RRID: AB_2888776 (BioLegend Cat. No. 310957)
AB_2888776 (BioLegend Cat. No. 310958)

Antigen Details

Structure: C-type lectin, type II glycoprotein, 28/32 kD
Distribution: Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
Function: Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type: B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
Gene ID: 969 View all products for this Gene ID
UniProt: View information about CD69 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD69 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD69	FN50	FC,CyTOF®,IHC-F
FITC anti-human CD69	FN50	FC
PE anti-human CD69	FN50	FC
PE/Cyanine5 anti-human CD69	FN50	FC
APC anti-human CD69	FN50	FC
APC/Cyanine7 anti-human CD69	FN50	FC
PE/Cyanine7 anti-human CD69	FN50	FC
Alexa Fluor® 488 anti-human CD69	FN50	FC
Alexa Fluor® 647 anti-human CD69	FN50	FC,SB
Pacific Blue™ anti-human CD69	FN50	FC
Alexa Fluor® 700 anti-human CD69	FN50	FC
Biotin anti-human CD69	FN50	FC
PerCP/Cyanine5.5 anti-human CD69	FN50	FC
PerCP anti-human CD69	FN50	FC
Brilliant Violet 421™ anti-human CD69	FN50	FC
Brilliant Violet 785™ anti-human CD69	FN50	FC
Brilliant Violet 650™ anti-human CD69	FN50	FC
Brilliant Violet 510™ anti-human CD69	FN50	FC
Brilliant Violet 605™ anti-human CD69	FN50	FC
Purified anti-human CD69 (Maxpar® Ready)	FN50	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD69	FN50	FC
Brilliant Violet 711™ anti-human CD69	FN50	FC
APC/Fire™ 750 anti-human CD69	FN50	FC
TotalSeq™-A0146 anti-human CD69	FN50	PG
TotalSeq™-B0146 anti-human CD69	FN50	PG
TotalSeq™-C0146 anti-human CD69	FN50	PG
Brilliant Violet 750™ anti-human CD69	FN50	FC
KIRAVIA Blue 520™ anti-human CD69	FN50	FC
Spark NIR™ 685 anti-human CD69 Antibody	FN50	FC
PE/Fire™ 640 anti-human CD69	FN50	FC
Spark YG™ 581 anti-human CD69	FN50	FC
TotalSeq™-D0146 anti-human CD69	FN50	PG
APC anti-human CD69	FN50	FC
Spark Blue™ 550 anti-human CD69	FN50	FC
PE anti-human CD69	FN50	FC
Spark Red™ 718 anti-human CD69	FN50	FC
GMP PE anti-human CD69	FN50	FC
PE/Fire™ 810 anti-human CD69	FN50	FC
PE/Fire™ 744 anti-human CD69	FN50	FC
Spark PLUS UV395™ anti-human CD69	FN50	FC
Spark Blue™ 574 anti-human CD69 (Flexi-Fluor™)	FN50	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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