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Spark YG™ 570 anti-human CD309 (VEGFR2) Antibody

Pricing & Availability
Clone
A16085I (See other available formats)
Regulatory Status
RUO
Other Names
VEGFR2, KDR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16085I_SparkYG570_CD309_Antibody_1_052120
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-buffered saline 1X (1.0 M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD309 (VEGFR2) (clone A16085I) Spark YG™ 570 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A16085I_SparkYG570_CD309_Antibody_1_052120
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-buffered saline 1X (1.0 M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD309 (VEGFR2) (clone A16085I) Spark YG™ 570 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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374005 25 µg 128€
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374006 100 µg 278€
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Description

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VEGFR2-Fc Chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2860956 (BioLegend Cat. No. 374005)
AB_2860956 (BioLegend Cat. No. 374006)

Antigen Details

Structure
Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution

Endothelial cells, embryonic tissues

Function
VEGFR2 regulates angiogenesis as well as vascular development and permeability
Interaction
VEGF-A, SHC2, NPR1, NCK1
Ligand/Receptor
VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. DiSalvo J, et al. 1995. J. Biol. Chem. 270:7717.
2. McColl BK, et al. 2003. J. Exp. Med. 198:863.
3. Albuquerque RJ, et al. 2009. Nat. Med. 15:1023.
4. Guangqi E, et al. 2012. J. Biol. Chem. 287:3029.
5. Koch S, and Claesson-Welsh 2012. Cold Spring Harb Perspect Med 2:a006502.
6. Munaut C, et al. 2012. Plos One 7:e33475.
7. Wehland M, et al. 2013. Int. J. Mol. Sci. 14:9338.

Gene ID
3791 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org
Go To Top Version: 1    Revision Date: 05/21/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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