- Clone
- 2H7 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV B201
- Other Names
- B1, Bp35
- Isotype
- Mouse IgG2b, κ
- Barcode Sequence
- TTCTGGGTCCCTAGA
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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302370 | 10 µg | 296€ |
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human tonsillar B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.
- Additional Product Notes
-
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes. -
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Polyak MJ, et al. 2002. Blood 99:3256. (IP)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- RRID
-
AB_2922531 (BioLegend Cat. No. 302370)
Antigen Details
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450. - Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Related FAQs
- What is the difference between two anti human CD20 clones 2H7 and 1412?
-
For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.
For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.
Our technical protocols can be found here.
Other Formats
View All CD20 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD20
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FITC anti-human CD20
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PE anti-human CD20
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PE/Cyanine5 anti-human CD20
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Purified anti-human CD20
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APC/Cyanine7 anti-human CD20
-
PE/Cyanine7 anti-human CD20
-
Alexa Fluor® 488 anti-human CD20
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Alexa Fluor® 647 anti-human CD20
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Pacific Blue™ anti-human CD20
-
Alexa Fluor® 700 anti-human CD20
-
PerCP anti-human CD20
-
PerCP/Cyanine5.5 anti-human CD20
-
Brilliant Violet 421™ anti-human CD20
-
Brilliant Violet 570™ anti-human CD20
-
Brilliant Violet 605™ anti-human CD20
-
Brilliant Violet 650™ anti-human CD20
-
Brilliant Violet 785™ anti-human CD20
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Brilliant Violet 510™ anti-human CD20
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Brilliant Violet 711™ anti-human CD20
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Purified anti-human CD20 (Maxpar® Ready)
-
PE/Dazzle™ 594 anti-human CD20
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Biotin anti-human CD20
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APC/Fire™ 750 anti-human CD20
-
Alexa Fluor® 594 anti-human CD20
-
FITC anti-human CD20
-
Pacific Blue™ anti-human CD20
-
APC anti-human CD20
-
PE/Cyanine7 anti-human CD20
-
TotalSeq™-A0100 anti-human CD20
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TotalSeq™-B0100 anti-human CD20
-
TotalSeq™-C0100 anti-human CD20
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PerCP/Cyanine5.5 anti-human CD20
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Spark NIR™ 685 anti-human CD20
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Spark YG™ 593 anti-human CD20
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GMP FITC anti-human CD20
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TotalSeq™-D0100 anti-human CD20
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GMP APC anti-human CD20
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Spark Violet™ 500 anti-human CD20
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GMP Pacific Blue™ anti-human CD20
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GMP PerCP/Cyanine5.5 anti-human CD20
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Spark Violet™ 538 anti-human CD20
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APC/Fire™ 750 anti-human CD20
-
PE anti-human CD20
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GMP PE/Cyanine7 anti-human CD20
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Spark Blue™ 515 anti-human CD20
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GMP APC/Fire™ 750 anti-human CD20
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GMP PE anti-human CD20
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Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™)
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Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™)
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Spark Red™ 718 anti-human CD20 (Flexi-Fluor™)
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APC/Fire™ 810 anti-human CD20
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Alexa Fluor® 660 anti-human CD20
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