- Clone
- HP-3G10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKR-P1A
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- GTACGCAGTCCTTCT
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
339953 | 10 µg | 296€ |
CD161 is a type II transmembrane glycoprotein, also known as NKR-P1A, that is expressed as a 40-44 kD homodimer. It is a member of the C-type lectin superfamily. CD161 is expressed on a majority of NK cells, NKT cells, and subsets of peripheral T cells and CD3+ thymocytes. It has been reported that Th17 cells are a subpopulation of CD4+CD161+CCR6+ cells. While the biological function of CD161 is not clear, it has been suggested to serve either as a stimulatory receptor or to inhibit NK cell-mediated cytotoxicity and cytokine production. LLT-1 (lectin-like transcript-1, also named as osteoclast inhibitory lectin or CLEC2D) is the ligand of CD161.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats) include: inhibition of cytokine production and Western blotting under nonreducing conditions.
- Additional Product Notes
-
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes. -
Application References
(PubMed link indicates BioLegend citation) -
- Gumß M, et al. 2004. Blood 104:3664.
- Exley M, et al. 1998. J. Exp. Med. 188:867.
- Marquez C, et al. 1998. Blood 91:2760.
- RRID
-
AB_2894602 (BioLegend Cat. No. 339953)
Antigen Details
- Structure
- Type II glycoprotein, 40-44 kD homodimer, C-type lectin superfamily
- Distribution
-
NK cells, T subset, subset of CD3+ thymocytes
- Ligand/Receptor
- LLT-1 (Lectin-like transcript-1)
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Takahashi T, et al. 2006. J. Immunol. 176:211.
2. Cosmi L, et al. 2008. J. Exp. Med. 205:1903.
3. Aldemir H, et al. 2005. J. Immunol. 175:7791.
4. Rosen DB, et al. 2008. J. Immunol. 180:6508. - Gene ID
- 3820 View all products for this Gene ID
- UniProt
- View information about CD161 on UniProt.org
Related FAQs
Other Formats
View All CD161 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 510™ anti-human CD161
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FITC anti-human CD161
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Purified anti-human CD161
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PE anti-human CD161
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PerCP/Cyanine5.5 anti-human CD161
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Alexa Fluor® 647 anti-human CD161
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APC anti-human CD161
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Brilliant Violet 421™ anti-human CD161
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Brilliant Violet 605™ anti-human CD161
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PE/Cyanine7 anti-human CD161
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Alexa Fluor® 700 anti-human CD161
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Purified anti-human CD161 (Maxpar® Ready)
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Alexa Fluor® 488 anti-human CD161
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Pacific Blue™ anti-human CD161
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APC/Cyanine7 anti-human CD161
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Brilliant Violet 785™ anti-human CD161
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Biotin anti-human CD161
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PerCP anti-human CD161
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PE/Dazzle™ 594 anti-human CD161
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APC/Fire™ 750 anti-human CD161
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TotalSeq™-A0149 anti-human CD161
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TotalSeq™-C0149 anti-human CD161
-
TotalSeq™-B0149 anti-human CD161
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PE/Cyanine5 anti-human CD161 Antibody
-
TotalSeq™-D0149 anti-human CD161
-
Spark Red™ 718 anti-human CD161
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Brilliant Violet 711™ anti-human CD161
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