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Ultra-LEAF™ Purified anti-human CD154 Antibody

Pricing & Availability
Clone
W20199I (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, TNFSF5, T-BAM, gp39
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20199I_ULEAF_CD154_FC_111924
Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail without Brefeldin A (Cat. No. 423302) for 6 hours. Cells were surface stained with Ultra-LEAF™ purified anti-human CD154 (clone W20199I) (left) or Ultra-LEAF™ purified rat IgG2b, κ isotype control, followed by goat anti-rat IgG PE. Cells were then co-stained with anti-human CD4 (clone RPA-T4) APC.
  • W20199I_ULEAF_CD154_FC_111924
    Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail without Brefeldin A (Cat. No. 423302) for 6 hours. Cells were surface stained with Ultra-LEAF™ purified anti-human CD154 (clone W20199I) (left) or Ultra-LEAF™ purified rat IgG2b, κ isotype control, followed by goat anti-rat IgG PE. Cells were then co-stained with anti-human CD4 (clone RPA-T4) APC.
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388205 100 µg 376€
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Description

CD154, also known as CD40L, TNFSF5, T-BAM, and gp39, is a 32-39 kD type II transmembrane glycoprotein member of the TNF superfamily expressed on activated T cells. CD154 interacts as a costimulatory molecule with CD40 on B Cells to induce B cell activation, resulting in proliferation, immunoglobulin production, and Ig Isotype switching. CD40L also may regulate T cell cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD40L protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
< 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W20199I is able to block the binding of clone 24-31 on target cells.

Clone W20199I is able to block the binding of CD40 and CD40L interaction on target cells.

Antigen Details

Structure
32-39 kD type II transmembrane glycoprotein of the TNF superfamily
Distribution

Activated T cells

Function
B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. van Kooten C and Banchereau J. 2000. J Leukoc Biol. 67:2-17.
  2. Ford GS, et al. 1999. J Immunol. 162:4037-44.
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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