- Clone
- P30-15 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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325223 | 100 µg | 216€ | ||||
325224 | 1 mg | 572€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The p30-15 monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells. NKp30 enhances NK cell cytolysis of tumor cellts that are deficient in MHC class I molecules. NKp30 has been shown to associate with CD59 and TCRζ. The p30-15 antibody against human NKp30 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human NKp30
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ELISA, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2814182 (BioLegend Cat. No. 325223)
AB_2814182 (BioLegend Cat. No. 325224)
Antigen Details
- Structure
- Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
- Distribution
-
IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro
- Function
- NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
- Interaction
- CD59, TCRζ
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Pende D, et al. 1999. J. Exp. Med. 190:1505.
- Gene ID
- 259197 View all products for this Gene ID
- UniProt
- View information about CD337 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD337 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with purified P30... -
Biotin anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with biotinylated... -
PE anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with P30-15 PE -
APC anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with P30-15 APC -
Alexa Fluor® 647 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with P30-15 Alexa... -
PerCP/Cyanine5.5 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD56 FI... -
PE/Cyanine7 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD337 (... -
Brilliant Violet 711™ anti-human CD337 (NKp30)
Human peripheral blood lymphocytes Brilliant Violet 711™ CD... -
TotalSeq™-C0801 anti-human CD337 (NKp30)
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TotalSeq™-A0801 anti-human CD337 (NKp30)
-
Brilliant Violet 785™ anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD56 FI... -
APC/Fire™ 750 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD56 FI... -
Brilliant Violet 421™ anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD56 FI... -
PE/Dazzle™ 594 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with CD56 FI... -
Ultra-LEAF™ Purified anti-human CD337 (NKp30)
Human peripheral blood lymphocytes stained with purified P30... -
TotalSeq™-B0801 anti-human CD337 (NKp30) Antibody
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Brilliant Violet 605™ anti-human CD337 (NKp30) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
KIRAVIA Blue 520™ anti-human CD337 (NKp30)
Human peripheral blood Lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0801 anti-human CD337 (NKp30)
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APC/Cyanine7 anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD337 (NKp30) (Flexi-Fluor™)
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