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Ultra-LEAF™ Purified anti-mouse CD255 (TWEAK) Antibody

Pricing & Availability
Clone
MTW-1 (See other available formats)
Regulatory Status
RUO
Other Names
TNFSF12, TNF-related weak inducer of apoptosis, CD255
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MTW-1_LEAF_090707
Mouse TWEAK transfected cells stained with Ultra-LEAF™ purified MTW-1, followed by biotinylated anti-rat IgG and Sav-PE
  • MTW-1_LEAF_090707
    Mouse TWEAK transfected cells stained with Ultra-LEAF™ purified MTW-1, followed by biotinylated anti-rat IgG and Sav-PE
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120009 100 µg 216€
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120010 1 mg 595€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

TWEAK is a 27 kD protein also known as tumor necrosis factor superfamily member 12 (TNFSF12), TNF-related weak inducer of apoptosis, and CD255. TWEAK is a type II transmembrane protein expressed on a variety of cell types. A soluble form of TWEAK is produced by proteolytic processing. TWEAK binds to the TWEAK receptor, FN14, to induce apoptosis, NF-κB translocation, and promote angiogenesis and endothelial cell proliferation. It has also been implicated in the induction of inflammatory cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of ligand binding2. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 120009 & 120010).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2003. Biochem. Biophys. Res. Commun. 306:819.
  2. Mittal A, et al. 2010. J. Cell Biol. 188:833. (Block) PubMed
RRID
AB_2860642 (BioLegend Cat. No. 120009)
AB_2860642 (BioLegend Cat. No. 120010)

Antigen Details

Structure
TNF superfamily member, type II membrane protein and secreted (after proteolytic processing); forms homotrimer, 27 kD
Distribution

Widely expressed; induced on activated CD4+ cells

Function
Weak inducer of apoptosis in some cell types, activates NF-κB, promotes angiogenesis and endothelial cell proliferation, induces inflammatory cytokine production
Ligand/Receptor
TWEAKR/FN14, possible interaction with TNFSF12/APO3
Cell Type
T cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Chicheportiche Y, et al. 1997. J. Biol. Chem. 272:32401.
2. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
3. Schneider P, et al. 1999. Eur. J. Immunol. 29:1785.

Gene ID
21944 View all products for this Gene ID
UniProt
View information about CD255 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05/01/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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