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Ultra-LEAF™ Purified anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
Clone
A18238E (See other available formats)
Regulatory Status
RUO
Other Names
CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A18238E_ULEAF_CD272_BTLA_Antibody_101921
Recombinant mouse HVEM-Fc chimera (Cat. No. 771306) (black circles) binds to immobilized recombinant mouse BTLA-Fc chimera (Cat. No. 775806). Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody (clone A18238E) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInViv™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody blocks the binding of 0.03 µg/mL recombinant mouse HVEM-Fc to 1 µg/mL immobilized recombinant mouse BTLA-Fc chimera. ND50 = 0.08 – 0.5 µg/mL.
  • A18238E_ULEAF_CD272_BTLA_Antibody_101921
    Recombinant mouse HVEM-Fc chimera (Cat. No. 771306) (black circles) binds to immobilized recombinant mouse BTLA-Fc chimera (Cat. No. 775806). Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody (clone A18238E) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInViv™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody blocks the binding of 0.03 µg/mL recombinant mouse HVEM-Fc to 1 µg/mL immobilized recombinant mouse BTLA-Fc chimera. ND50 = 0.08 – 0.5 µg/mL.
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941803 100 µg 269€
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941804 1 mg 836€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

BTLA (B and T lymphocyte attenuator, CD272) is a type I transmembrane glycoprotein with four extracellular cysteine-rich domains. It is an inhibitory molecule structurally and functionally similar to cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1). These molecules belong to the immunoglobulin superfamily that binds B7 family members. Different from CTLA-4 and PD-1 binding with the B7 family members, BTLA interacts with herpes virus entry mediator (HVEM) which belongs to TNFR family. BTLA/HVEM interaction was the first demonstration of crosstalk between these two family members. BTLA/HVEM interaction negatively modulates T cell activation. HVEM induces BTLA tyrosine phosphorylation and inhibits T-cell proliferation. There is accumulating evidence suggesting that BTLA/HVEM signaling also plays an important role in autoimmunity and infection at mucosal surfaces. The BTLA/HVEM interaction does not prevent the simultaneous binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and natural killer cells. Constitutive expression level of BTLA on resting T cells are very low, but increase after the activation of T cells. BTLA is highly expressed on resting B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD272 (BTLA)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between mouse BTLA-Fc chimera and mouse HVEM-Fc chimera. Ultra-LEAFTM purified anti-mouse CD272 (BTLA) antibody blocks the binding of 0.03 µg/mL recombinant mouse HVEM-Fc chimera to 1 µg/mL immobilized recombinant mouse BTLA-Fc chimera. ND50 range: 0.08 – 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904452 (BioLegend Cat. No. 941803)
AB_2904452 (BioLegend Cat. No. 941804)

Antigen Details

Structure
An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4
Distribution

Lymphocytes, T cells, B cells, macrophages, dendritic cells, NKT cells and natural killer cells.

Function
Negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM)
Interaction
Lymphocytes
Ligand/Receptor
HVEM
Cell Type
B cells, Dendritic cells, Lymphocytes, Macrophages, NK cells, NKT cells, T cells
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
Immune Checkpoint Receptors
Antigen References
  1. Watanabe N, et al. 2003. Nat. Immunol. 4: 670-9
  2. Gavrieli M, et al. 2003. Biochem. Biophys. Res. Commun. 312: 1236-43 
  3. Gonzalez LC, et al. 2005. Proc. Natl. Acad. Sci. 102: 1116-21
  4. Sedy JR, et al. 2005. Nat. Immunol. 6: 90-8
  5. Murphy KM, et al. 2006. Nat. Rev. Immunol. 6: 671-81
Gene ID
208154 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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