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Ultra-LEAF™ Purified anti-mouse IL-33Rα (ST2) Antibody

Pricing & Availability
Clone
DIH4 (See other available formats)
Regulatory Status
RUO
Other Names
ST2, ST2L, IL-1R1
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
a_DIH4_LEAF_IL-33RaST2 _Antibody_1_102813.jpg
Mouse Th2 clone D10.G4.1 was stained with LEAF™ purified anti-mouse IL-33Rα (ST2) (clone DIH4, filled histogram) or rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • a_DIH4_LEAF_IL-33RaST2 _Antibody_1_102813.jpg
    Mouse Th2 clone D10.G4.1 was stained with LEAF™ purified anti-mouse IL-33Rα (ST2) (clone DIH4, filled histogram) or rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • DIH4_LEAF_IL-33Ra_Antibody_Block_2_061014
    Recombinant mouse IL-33 mediated D10.G4.1 proliferation inhibited by anti-mouse IL-33Rα (ST2) (clone DIH4).
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146604 500 µg 282€
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Description

IL-33Ra, also known as ST2 or IL-1RL1 is a member of the Toll/IL-1 receptor family. It binds IL-33 and is structurally similar to IL-1R1. Two forms of the protein exist – soluble form known as ST2 and membrane anchored form known as ST2L. The membrane form is expressed by Th2 cells and bone marrow derived mast cells whereas the soluble form is expressed by serum-stimulated fibroblasts.

Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation. The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies and cardiac disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-33Rα-hFcγ 1 fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC, Block) PubMed
Product Citations
  1. Hoppe A, et al. 2020. J Allergy Clin Immunol. 1476:145. PubMed
  2. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
RRID
AB_2562656 (BioLegend Cat. No. 146604)

Antigen Details

Structure
Approximately 63kD, IL-1 receptor family member, single pass type I membrane protein
Distribution

Mast cells, Th2 cells and fibroblasts

Function
Interacts with IL-33 and recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway
Interaction
Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor
IL-33
Cell Type
Mast cells, Th2, Fibroblasts
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
  2. Schmitt E, et al. 1990. Cytokine. 6:407.
  3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
  4. Takagi T, et al. 1993. Biochim. Biophys. Acta. 1178:194.
Gene ID
17082 View all products for this Gene ID
UniProt
View information about IL-33Ralpha on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 06/10/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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