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Ultra-LEAF™ Purified anti-mouse/rat TNF-α Antibody

Pricing & Availability
Clone
TN3-19.12 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype
Armenian Hamster IgG
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Product Citations
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Cat # Size Price Quantity Check Availability Save
506112 25 mg 1127€
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506113 50 mg 1884€
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506114 100 mg 2707€
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506109 100 µg 88€
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506110 1 mg 120€
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506111 5 mg 432€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-α (TNF-α) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Reported Reactivity
Rabbit
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, ICFC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-a. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Clone TN3-19.12 has been shown to neutralize murine TNF-a and cross reacts wtih mouse TFN-ß1. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Aside free, 0.2 µm filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan, K., et al. 1989. J. Immunol. 142:3884. (Neut)
  2. Merrick, B.A., et al. 1992. Appl. Theor. Electrophor 2:177.
  3. Takahashi, S., et al. 1995. Brain Res 690:241.
  4. Rabinovich, R., et al. 1993. J. Pharmacol. Exp. Ther. 267:1550.
  5. Molloy, R., et al. 1993. J. Immunol. 151:2142.
  6. Finkelman, F., et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Simkins, HM., et al. 2011. J. Leukoc Biol. 89:753. PubMed.
  8. Faas MM, et al. 2003. J. Reprod. Immunol. 1:39-52.
Product Citations
  1. Tseng JC, et al. 2023. Int J Biol Sci. 19:2648. PubMed
RRID
AB_2832813 (BioLegend Cat. No. 506112)
AB_2832813 (BioLegend Cat. No. 506113)
AB_2832813 (BioLegend Cat. No. 506114)
AB_2832813 (BioLegend Cat. No. 506109)
AB_2832813 (BioLegend Cat. No. 506110)
AB_2832813 (BioLegend Cat. No. 506111)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID
21926 View all products for this Gene ID 24835 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All TNF-α Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse / rat TNF-α TN3-19.12 ICFC
Purified anti-mouse / rat TNF-α TN3-19.12 ELISA Capture,IP,WB
APC anti-mouse/rat TNF-α TN3-19.12 ICFC
Ultra-LEAF™ Purified anti-mouse/rat TNF-α TN3-19.12 ELISA Capture,ELISPOT Capture,ICFC,IP,Neut,WB
Go To Top Version: 1    Revision Date: 11/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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