Ultra-LEAF™ Purified anti-human VISTA (PD-1H) Antibody

Pricing & Availability
Clone
A17002E (See other available formats)
Regulatory Status
RUO
Other Names
V-Set Immunoregulatory Receptor (VSIR), V-Domain Ig Suppressor of T Cell Activation (VISTA), Stress-Induced Secreted Protein-1 (SISP1), PDCD1 Homolog, Platelet Receptor GI24 (GI24), Death Domain1alpha, DD1 alpha, B7-H5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A17002E_ULEAF_VISTA_Antibody_121622
Human peripheral blood monocytes were stained with Ultra-LEAF™ purified anti-human VISTA (PD-1H) (clone A17002E) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • A17002E_ULEAF_VISTA_Antibody_121622
    Human peripheral blood monocytes were stained with Ultra-LEAF™ purified anti-human VISTA (PD-1H) (clone A17002E) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
Cat # Size Price Quantity Check Availability Save
380003 100 µg 216€
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Description

VISTA (V-domain Ig suppressor of T cell activation), also known as PD-1H, is a type I transmembrane protein with a single extracellular IgV domain. VISTA is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It was reported that in certain tumor environment, there is low-pH dependent interaction between VISTA and PSGL1. However, the physiological binding partner for VISTA and the mechanism of its action are not clearly understood. VISTA is considered as a checkpoint regulator and a promising target for cancer immunotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human VISTA protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A17002E can completely block the binding of clone A17002B on target cells. 

Clone A17002E can reduce T cell proliferation in an in vitro T cell activation assay.

RRID
AB_2928033 (BioLegend Cat. No. 380003)

Antigen Details

Structure
Ig V-domain like
Distribution

Hematopoietic cells, monocytes, macrophages, dendritic cell, granulocytes, subsets of T cells

Function
Suppressing T cell activation
Cell Type
Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References
  1. Wang Li, et al. 2011. J Exp Med. 208:577-92.
  2. Nowak EC, et al. 2017. Immunol Rev. 276:66-79.
  3. Flies DB, et al. 2014. J Clin Invest. 124:1966-75.
Gene ID
64115 View all products for this Gene ID
UniProt
View information about VISTA on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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