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APC anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_16B12_APC_HA11_Antibody_071923
Transfected RBL1 cells expressing the HA tag on the cell surface were stained with anti-HA.11 Eepitope Tag (clone 16B12) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • A_16B12_APC_HA11_Antibody_071923
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with anti-HA.11 Eepitope Tag (clone 16B12) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • B_16B12_APC_HAdot11_EpitopeTag_Antibody_051518
    CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly stained with HA.11 Epitope Tag (16B12) APC.
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901523 25 µg 138€
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901524 100 µg 356€
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For intracellular flow cytometric staining using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
  13. Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
  14. Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
  15. Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
  16. Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
  17. Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
  18. Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
  19. Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
  20. Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
  21. Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
  22. Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
  23. Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
  24. Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
  25. Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
  26. Lee B, et al. 2016. Development. 143:1721-31. PubMed
  27. Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
  28. Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
  29. Shin H, et al. 2015. Nature. 534:553-7. PubMed
  30. Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
  31. Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
  32. Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
  33. Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
  34. Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
  35. Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
  36. Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
  37. Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
  38. Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
  39. Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
  40. Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
  41. Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
  42. Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
  43. Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
  44. Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
  45. Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
  46. Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
  47. Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
  48. Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
  49. Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
  50. Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
  51. Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
  52. Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
  53. Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
  54. Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
  55. Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
  56. Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
  57. Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
  58. Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
  59. Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
  60. Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
  61. Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
  62. Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
  63. Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
  64. Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
  65. Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
  66. Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
  67. Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
  68. Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
  69. Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
  70. Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
  71. Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
  72. Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
  73. Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
  74. K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
  75. Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
  76. Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
  77. T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
  78. Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
  79. Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
  80. Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed
  81. Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
  82. Daniel JA, et al. 2017. Elife. 6:e26338. PubMed
  83. Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
Product Citations
  1. Veit G, et al. 2020. JCI Insight. 5:00. PubMed
  2. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  3. Veit G, et al. 2021. J Pers Med. 11:. PubMed
  4. Omarjee O, et al. 2021. J Exp Med. 218:. PubMed
  5. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  6. Liu H, et al. 2022. Commun Biol. 5:766. PubMed
  7. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  8. Fujiwara K, et al. 2020. Cancers (Basel). 12:. PubMed
  9. Salgado–Polo F, et al. 2020. J Cell Sci. 133:jcs235044. PubMed
  10. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
RRID
AB_2734657 (BioLegend Cat. No. 901523)
AB_2734657 (BioLegend Cat. No. 901524)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org
Go To Top Version: 3    Revision Date: 07/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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