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APC anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10Fdot9G2_APC_040610
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
  • 10Fdot9G2_APC_040610
    C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
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124311 25 µg 71€
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124312 100 µg 216€
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Ngiow S, et al. 2016. Cancer Res . 76: 6266 - 6277. PubMed
  2. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  3. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  4. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  5. Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed
  6. Wang X, et al. 2022. Sci Adv. 8:eabn0071. PubMed
  7. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  8. An HW, et al. 2022. Cell Rep. 41:111878. PubMed
  9. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  10. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  11. Chen H, et al. 2023. Nat Commun. 14:941. PubMed
  12. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  13. Zhang X, et al. 2023. Heliyon. 9:e13490. PubMed
  14. Tsai KC, et al. 2023. Mar Drugs. 21:. PubMed
  15. Gu T, et al. 2023. Front Immunol. 14:1145028. PubMed
  16. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  17. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  18. Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed
  19. Tatsuguchi T, et al. 2022. Int Immunol. 34:277. PubMed
  20. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  21. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  23. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  24. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  25. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  26. Park S, et al. 2021. Front Immunol. 11:620166. PubMed
  27. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  28. Chen L, et al. 2020. Cancer Immunol Res. 0.457638889. PubMed
  29. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  30. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  31. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  32. Ren J, et al. 2021. Theranostics. 11:304. PubMed
  33. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  34. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  35. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  36. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  37. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  38. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  39. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  40. Zhou KX, et al. 2021. J Immunol Res. 2021:6694392. PubMed
  41. Tu J, et al. 2022. Theranostics. 12:747. PubMed
  42. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  43. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  44. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  45. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  46. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  47. Poon E, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0268-8. PubMed
  48. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  49. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  50. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  51. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  52. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  53. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  54. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  55. Huang L, et al. 2021. Breast Cancer Res Treat. Online ahead of print. PubMed
  56. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  57. Fukumoto T, et al. 2019. Cancer Res. 79:5482. PubMed
  58. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  59. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  60. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  61. Shen ZY, et al. 2021. Front Immunol. 12:589200. PubMed
  62. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  63. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  64. Franklin DA, et al. 2020. JCI Insight. 5:. PubMed
  65. Baldwin LA, et al. 2022. Nat Commun. 13:6539. PubMed
  66. Yi M, et al. 2022. J Hematol Oncol. 15:142. PubMed
  67. Li Q, et al. 2022. Front Oncol. 12:841977. PubMed
  68. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  69. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  70. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  71. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  72. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  73. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  74. Wang C, et al. 2018. Sci Transl Med. 10. PubMed
  75. Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
  76. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  77. Lin J, et al. 2021. Cancer Immunol Res. 9:1413. PubMed
  78. Liu YS, et al. 2021. Cancers (Basel). 13:. PubMed
  79. Luna-Yolba R, et al. 2021. Cancers (Basel). 13: . PubMed
  80. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  81. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  82. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  83. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
  84. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  85. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID
AB_10612935 (BioLegend Cat. No. 124311)
AB_10612935 (BioLegend Cat. No. 124312)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 2    Revision Date: 12/22/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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