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APC anti-mouse Notch 4 Antibody

Pricing & Availability
Clone
HMN4-14 (See other available formats)
Regulatory Status
RUO
Other Names
Proto-oncogene of int-3
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMN4-14_APC_092308.jpg
FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 APC
  • HMN4-14_APC_092308.jpg
    FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 APC
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128413 25 µg 129€
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Description

The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligands identified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotes differentiation of B lymphocytes to antibody-secreting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Notch4-Fc recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al.2008. Intl J. Immunol. 20:763.
  2. Reilla LV, et al. 2011. J. Immunol. 187:4629. PubMed.
Product Citations
  1. Pribluda A, et al. 2022. Elife. 11: . PubMed
  2. Riella L, et al. 2011. J Immunol. 187:4629. PubMed
RRID
AB_2151253 (BioLegend Cat. No. 128413)

Antigen Details

Structure
Type 1 transmembrane receptor with a large extracellular domain consisting of 29 -36 EGF( epidermal growth factor) and three LNR (Lin12-Notch) repeats.
Distribution

Notch 4 is preferentially expressed in endothelial cells.

Function
Regulation of cell development, including myogenesis, neurogenesis, and lymphocyte development. Notch 4 seems to play a crucial role in vasculogenesis and angiogenesis.
Ligand/Receptor
Jagged 1, Jagged2, Delta-like 1, Delta-like3 and Delta-like 4
Cell Type
Endothelial cells
Biology Area
Immunology
Antigen References

1.Ehebauer MT,et al. 2006. Biochem J.392:13.
2.Shimizu K,et al. 2000.Mol Cell Biology. 20:18
3. Tanigaki K,et al.2007. Nature immunol. 8:451.

Gene ID
18132 View all products for this Gene ID
UniProt
View information about Notch 4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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