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APC/Cyanine7 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_APCCyanine7_CD46_Antibody_062119
Human peripheral blood monocytes stained with CD14 (clone HCD14) APC/Cyanine7 (filled histogram), or Mouse IgG1, κ APC/Cyanine7 isotype (open histogram).
  • HCD14_APCCyanine7_CD46_Antibody_062119
    Human peripheral blood monocytes stained with CD14 (clone HCD14) APC/Cyanine7 (filled histogram), or Mouse IgG1, κ APC/Cyanine7 isotype (open histogram).
Compare all formats See APC/Cyanine7 spectral data
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325619 25 tests 123€
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325620 100 tests 249€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  2. Carre C, et al. 2021. iScience. 24:102970. PubMed
  3. Sefik E, et al. 2022. Nature. 606:585. PubMed
  4. Sungalee S, et al. 2021. Nat Genet. 53:650. PubMed
  5. Beitnes AC, et al. 2012. PLoS One. 7:e33556. PubMed
  6. Sun R, et al. 2021. Cell Reports. 36(7):109559. PubMed
  7. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  8. Hilliard S, et al. 2023. iScience. 26:106041. PubMed
  9. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  10. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  11. Rajasekhar M, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.01.002. PubMed
  12. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  13. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  14. Tiwari-Heckler S, et al. 2021. Cell Rep. 37:109897. PubMed
  15. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  16. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  17. Soriano-Sarabia N, et al. 2012. J Immunol. 189:5212. PubMed
  18. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  19. Hagen SH, et al. 2020. Cell Rep. 33:108485. PubMed
  20. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  21. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  22. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  23. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  24. Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
  25. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  26. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  27. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  28. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  29. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  30. Markman JL, et al. 2020. Nat Commun. 11:1613. PubMed
  31. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  32. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  33. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  34. Port JR, et al. 2020. J Virol. 94:. PubMed
  35. Zhuang Q, et al. 2021. Front Pharmacol. 12:706580. PubMed
  36. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  37. Hagen SH, et al. 2021. STAR Protocols. 2(3):100641. PubMed
  38. Bravo-Flores E, et al. 2018. Int J Mol Sci. 19:E1074. PubMed
  39. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  40. Nakajima S, et al. 2021. Mol Cancer Res. 19:1085. PubMed
  41. Ravenhill BJ, et al. 2020. Sci Rep. 10:4560. PubMed
  42. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  43. He L, et al. 2022. EMBO Mol Med. 14:e14990. PubMed
  44. Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed
  45. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  46. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  47. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
RRID
AB_830692 (BioLegend Cat. No. 325619)
AB_830692 (BioLegend Cat. No. 325620)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 3    Revision Date: 06/21/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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