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Brilliant Violet 421™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_BV421_1_062911
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_1_062911
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_2_062911
  • 17A2_BV421_CD3_Antibody_IF_012914
    C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 17A2) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
100227 125 µL 141€
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100228 50 µg 194€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kalinina A, et al. 2021. STAR Protoc. 2:100368. PubMed
  2. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  3. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  4. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  5. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  6. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  7. Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed
  8. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  9. Liu H, et al. 2023. Breast Cancer Res. 25:43. PubMed
  10. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  11. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  12. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  13. Chukkapalli S, et al. 2015. Pathog Dis. 73:9. PubMed
  14. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  15. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  16. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  17. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  18. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  19. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  20. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  21. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  22. Trenevska I, et al. 2021. PLoS One. 16:e0249967. PubMed
  23. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  24. Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
  25. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  26. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  27. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  28. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  29. Glaser AK, et al. 2019. Nat Commun. 10:2781. PubMed
  30. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  31. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  32. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  33. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  34. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  35. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  36. Chukkapalli S, et al. 2015. PLoS One. 10: 0143291. PubMed
  37. Jin C, et al. 2019. Cell. 176:998. PubMed
  38. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  39. Bristow CL, et al. 2021. Front Oncol. 11:739080. PubMed
  40. Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed
  41. Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed
  42. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  43. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  44. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  45. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  46. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  47. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  48. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  49. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
RRID
AB_2562553 (BioLegend Cat. No. 100227)
AB_2562553 (BioLegend Cat. No. 100228)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 01/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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