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PerCP/Cyanine5.5 anti-human CD8a Antibody

Pricing & Availability
Clone
HIT8a (See other available formats)
Regulatory Status
RUO
Workshop
V CD08.10
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIT8a_PerCPCyanine55_CD8_Antibody_060619_updated.png
Human peripheral blood lymphocytes stained with CD8 (clone HIT8a) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
  • HIT8a_PerCPCyanine55_CD8_Antibody_060619_updated.png
    Human peripheral blood lymphocytes stained with CD8 (clone HIT8a) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
Compare all formats See PerCP/Cyanine5.5 spectral data
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300923 25 tests 141€
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300924 100 tests 306€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Common Marmoset
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
  5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
  6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
Product Citations
  1. Wang CQ, et al. 2023. Cells. 12: . PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  4. Hu X, et al. 2023. Nat Commun. 14:2020. PubMed
  5. Balakrishnan A, et al. 2017. Biol Blood Marrow Transplant. 10.1016/j.bbmt.2017.07.016. PubMed
  6. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  7. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  8. Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
  9. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  10. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  11. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  12. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  13. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  14. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  15. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  16. Keskin DB, et al. 2019. Nature. 565:234. PubMed
RRID
AB_1575079 (BioLegend Cat. No. 300923)
AB_1575079 (BioLegend Cat. No. 300924)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 6    Revision Date: 06/06/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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