- Clone
- W20045D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Excision Repair Cross-Complementation Group 1, ERCC excision repair 1, endonuclease non-catalytic subunit, RAD10
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
947501 | 25 µg | 95€ | ||||
947502 | 100 µg | 235€ |
ERCC1, also referred to as Excision Repair Cross-Complementation Group 1, is a component of the nucleotide excision repair pathway. ERCC1 forms a heterodimer with XPF endonuclease to catalyze the 5’ incision in the process of excising a DNA lesion. ERCC1 also works with SLX4 in homology direct repair of double strand breaks and in the repair of crosslinks. Mutations in ERCC1 play a role in carcinogenesis due to genome instability, and elevated expression of ERCC1 is predictive of resistance to platinum-based chemotherapies in multiple types of cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human ERCC1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.625 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W20045D detects multiple ERCC1 isoforms by western blot.
Clone W20045D was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with ERCC1 staining. - RRID
-
AB_2894534 (BioLegend Cat. No. 947501)
AB_2894534 (BioLegend Cat. No. 947502)
Antigen Details
- Structure
- ERCC1 is a 297 amino acid protein with an expected molecular weight of 33 kD. Four isoforms between 25 and 36 kD have been described.
- Distribution
-
Ubiquitously expressed/ Nucleus
- Function
- DNA damage repair
- Biology Area
- Cell Biology, DNA Repair/Replication
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Friboulet, et al. 2013. Cell Cycle. 12:3298
- Kashiyama, et al. 2013. J Hum Genet. 92:807
- Wan J, et al. 2017. Canc Invest. 35:85
- Faridounnia, et al. 2018. Molecules. 23:3205
- Gene ID
- 2067 View all products for this Gene ID
- UniProt
- View information about ERCC1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All ERCC1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ERCC1 | W20045D | WB,IHC-P,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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