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Purified anti-human CD340 (erbB2/HER-2) Antibody

Pricing & Availability
Clone
24D2 (See other available formats)
Regulatory Status
RUO
Other Names
HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
24D2_Purified_072607
Human breast cancer cell line (MCF-7) stained with purified 24D2, followed by anti-mouse IgG FITC
  • 24D2_Purified_072607
    Human breast cancer cell line (MCF-7) stained with purified 24D2, followed by anti-mouse IgG FITC
  • z24D2_IF_072512
    BT474 breast cancer cells were stained with anti-CD340 (clone 24D2) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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324401 25 µg 81€
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324402 100 µg 165€
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Description

CD340 is also known as HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, and oncogene NGL, neuroblastoma- or glioblastoma-derived. It is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain and has an approximate molecular weight of 185 kD. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region (ECR). The HER2/erbB2 protein is expressed on many tumor cells, including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow mesenchymal stem cells. Although the erbB2/HER2 protein contains no ligand binding domain, this protein interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. The erbB2/HER-2 protein has been shown to interact with a large number of proteins, including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others. CD340 is extensively modified by tyrosine phosphorylation on multiple residues (Y1023, Y1139, Y1196, Y1221, Y1222, Y1248).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 transfected with human HER-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Bühring HJ, et al. 1995. Blood 86:1916.
Product Citations
  1. Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed
  2. Cahall CF, et al. 2015. Breast Cancer (Auckl). 1:9. PubMed
  3. Rasoulouniriana D, et al. 2023. Cancer Immunol Res. 792:11. PubMed
  4. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  5. Rau A, et al. 2021. MAbs. 1902034:13. PubMed
  6. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  7. Huang CH, et al. 2019. ACS Nano. 13:11698. PubMed
  8. Luan H, et al. 2021. Cancers (Basel). 13:. PubMed
RRID
AB_756118 (BioLegend Cat. No. 324401)
AB_756118 (BioLegend Cat. No. 324402)

Antigen Details

Structure
Member of the epidermal growth factor receptor family of cell membrane tyrosine kinases. Single transmembrane pass protein with an approximate molecular weight of 185 kD. Contains three furin repeats and two cheY homologous receiver domains in the ECR.
Distribution

Expressed on many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow MSCs.

Function
Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
Interaction
CD340 (HER2/erbB2) interacts with a large number of proteins, including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Modification
Extensively modified by tyrosine phosphoryation on multiple residues (Y1023, Y1139, Y1196, Y1221, Y1222, Y1248).
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Akiyama T, et al. 1986. Science 232:1644.
2. Bargmann CI, et al. 1986. Nature 319:226.
3. Pegram MD, et al. 1997. Oncogene 15:537.
4. Slamon DJ, et al. 1989. Science 244:707.
5. Leone F, et al. 2003. J. Leukocyte Biol. 74:593.

Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org
Go To Top Version: 2    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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