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Purified anti-human HLA-DR, DP, DQ Antibody

Pricing & Availability
Clone
Tü39 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
a-Tu39_Purified_HLA-DR_Antibody_FC_012314.jpg
Human peripheral blood lymphocytes were stained with purified HLA-DR, DP, DQ (clone Tü39) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • a-Tu39_Purified_HLA-DR_Antibody_FC_012314.jpg
    Human peripheral blood lymphocytes were stained with purified HLA-DR, DP, DQ (clone Tü39) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • b-Tu39_Purified_HLA-DR_Antibody_IHC_121622
    IHC staining of Purified anti-human HLA-DR, DP, DQ (clone Tü39) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20 (Cat. No. 925501), the tissue was incubated without (panel A) and with (panel B) 5.0 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
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361702 100 µg 109€
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Description

HLA-DR, HLA-DP, and HLA-DQ are heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Cat, Dog, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IP- Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Tü39 has been reported to react with a shared epitope of HLA-DR, HLA-DP, and HLA-DQ.

Additional reported applications (of relevant formats) include immunoprecipitation6, in vitro blocking of MLR5, and suppressor cell generation4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Pawelec G, et al. 1985. Hum. Immunol. 12:165.
  2. Shaw S, et al. 1985. Hum. Immunol. 12:191.
  3. Ziegler A, et al. 1986. Immunobiology 171:77.
  4. Pawelec G, et al. 1986. Hum. Immunol. 17:343. (Block)
  5. Dai Z, et al. 2009. J. Exp. Med. 206:793. (Block)
  6. Pawelec G, et al. 1988. J. Exp. Med. 167:243. (IP)
Product Citations
  1. Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed
  2. Gallen C, et al. 2022. Vaccines (Basel). 10:. PubMed
  3. Lin W, et al. 2023. Commun Biol. 6:447. PubMed
  4. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  5. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  6. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  7. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  8. Donia M, et al. 2015. Cancer Res . 75: 3747-3759. PubMed
RRID
AB_2563139 (BioLegend Cat. No. 361702)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol Rev. 85:5.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID 3117 View all products for this Gene ID 3119 View all products for this Gene ID
UniProt
View information about HLA-DR DP DQ on UniProt.org
Go To Top Version: 4    Revision Date: 12/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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