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Purified anti-human IL-6 Antibody

Pricing & Availability
Clone
MQ2-13A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
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Product Citations
publications
HumanIL-6_061604
  • HumanIL-6_061604
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501101 50 µg 67€
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501102 500 µg 191€
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, Neut, ICFC - Reported in literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry10.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
  4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Zou JP, et al. 1999. J. Immunol. 162:4882.
  6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
  7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
  8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
  9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  2. Terasaka Y, et al. 2010. Invest Ophthalmol Vis Sci. 51:2441. PubMed
  3. Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
  4. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  5. Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
RRID
AB_315150 (BioLegend Cat. No. 501101)
AB_315150 (BioLegend Cat. No. 501102)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human IL-6 MQ2-13A5 ICFC
PE anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
APC anti-human IL-6 MQ2-13A5 ICFC
Pacific Blue™ anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 (Maxpar® Ready) MQ2-13A5 ELISA Capture,CyTOF®
PerCP/Cyanine5.5 anti-human IL-6 MQ2-13A5 ICFC
PE/Cyanine7 anti-human IL-6 MQ2-13A5 ICFC
Alexa Fluor® 647 anti-human IL-6 MQ2-13A5 ICFC
PE/Dazzle™ 594 anti-human IL-6 MQ2-13A5 ICFC
Ultra-LEAF™ Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
TotalSeq™-B0942 anti-human IL-6 MQ2-13A5 ICPG
TotalSeq™-C0942 anti-human IL-6 MQ2-13A5 ICPG
Go To Top Version: 4    Revision Date: 06/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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