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Purified anti-human TCR Vα24-Jα18 (iNKT cell) Antibody

Pricing & Availability
Clone
6B11 (See other available formats)
Regulatory Status
RUO
Other Names
TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_6B11_PE_070609.jpg
Human peripheral blood lymphocytes stained with purified 6B11 conjugated to PE and CD3 (UCHT1) Alexa Fluor® 647 (top) or mIgG1, κ (MOPC-21) PE isotype control and CD3 (UCHT1) Alexa Fluor® 647 (bottom)
  • A_6B11_PE_070609.jpg
    Human peripheral blood lymphocytes stained with purified 6B11 conjugated to PE and CD3 (UCHT1) Alexa Fluor® 647 (top) or mIgG1, κ (MOPC-21) PE isotype control and CD3 (UCHT1) Alexa Fluor® 647 (bottom)
  • B_6B11_PE_070609.jpg
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342902 100 µg 100€
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Description

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.

Application References

(PubMed link indicates BioLegend citation)
  1. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Product Citations
  1. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  2. Nugent JL, et al. 2021. iScience. 24:103421. PubMed
  3. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  4. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  5. Schwabenland M, et al. 2021. Immunity. . PubMed
  6. Melo AM, et al. 2020. Cancer Immunol Immunother. 69:969. PubMed
  7. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_2229301 (BioLegend Cat. No. 342902)

Antigen Details

Structure
Invariant CDR3 region of TCR Vα24-JαQ
Distribution

Invariant NKT cells

Ligand/Receptor
Glycolipid loaded CD1d
Cell Type
NKT cells
Biology Area
Immunology
Molecular Family
TCRs
Antigen References
  1. Thomas SY, et al. 2003. J. Immunol. 171:2571.
  2. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
  3. Montoya CJ, et al. 2007. Immunology. 122:1.
  4. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID
28659 View all products for this Gene ID
UniProt
View information about TCR Valpha24-Jalpha18 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TCR Vα24-Jα18 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PE anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
FITC anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
APC anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PE/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PerCP/Cyanine5.5 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
Brilliant Violet 421™ anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
Brilliant Violet 510™ anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PE/Dazzle™ 594 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
Brilliant Violet 711™ anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
TotalSeq™-A0584 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 PG
TotalSeq™-C0584 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 PG
Brilliant Violet 605™ anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
Brilliant Violet 785™ anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
TotalSeq™-B0584 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 PG
APC/Fire™ 750 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
APC/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PerCP/Fire™ 780 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
PerCP/Fire™ 806 anti-human TCR Vα24-Jα18 (iNKT cell) 6B11 FC
Spark Red™ 718 anti-human TCR Vα24-Jα18 (Flexi-Fluor™) 6B11 FC
Spark Blue™ 574 anti-human TCR Vα24-Jα18 (Flexi-Fluor™) 6B11 FC
Spark Blue™ 550 anti-human TCR Vα24-Jα18 (Flexi-Fluor™) 6B11 FC
Go To Top Version: 3    Revision Date: 08/30/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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