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A single cell suspension from C57BL/6 mouse spleen was prepared for the positive selection of CD8a+ cells using the MojoSort™ Mouse CD8a Selection Kit. Cells were stained with anti-mouse CD8b (clone YTS156.7.7) PE and anti-mouse CD3 (clone 17A2) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: CD8a+ cells after isolation. Right plot: Cells before isolation.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480135 | 20 tests | 165€ | ||||
| 480136 | 200 tests | 566€ | ||||
Mouse CD8a+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD8a antibody and then magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD8a+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480135:
- 1 vial containing 100 µL of Biotin anti-mouse CD8a antibody (100X) (clone 53-6.7)
- 1 vial containing 200 µL of Streptavidin Nanobeads
For Cat# 480136:
- 1 vial containing 1 mL of Biotin anti-mouse CD8a antibody (100x) (clone 53-6.7)
- 2 vials containing 1 mL each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
- The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Streptavidin Nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
- Recommended Usage
-
10 µL of pre-diluted Biotin anti-mouse CD8a antibody for 1 x 107 cells in 100 µL of buffer. Note: Prepare the pre-diluted antibody by adding 5 µL of the Biotin anti-mouse CD8a (100X) antibody to 495 µL 1X MojoSort Buffer*.
*Suggested 100X dilution volumes results in excess diluted antibody per test. We discourage performing a 100X dilution using smaller volumes of stock antibody (e.g. 1 µL antibody + 99 µL Buffer) due to increased likelihood for pipetting error and affecting cell separation results.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer - Application Notes
-
This kit is designed for the positive selection or depletion of mouse CD8a+ cells from mouse splenocytes.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 200X
Nanobead dilution to use in columns: 4X - Product Citations
-
Antigen Details
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- CD8a
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD8a
- App Abbreviation (DOES NOT SHOW ON TDS):
- Cell Sep - Pos
- UniProt
- View information about CD8a on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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