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PE anti-human CD45 Antibody

Pricing & Availability
Clone
2D1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte Common Antigen (LCA), T200
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2D1_PE_CD45_Antibody_FC_102215
Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 2D1_PE_CD45_Antibody_FC_102215
    Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
368509 25 tests 49€
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368510 100 tests 111€
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Description

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References
  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations
  1. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  2. Sasamoto Y, et al. 2022. Cell Rep. 40:111166. PubMed
  3. Ding L, et al. 2023. J Clin Invest. 133: . PubMed
  4. Cillo AR, et al. 2022. Clin Cancer Res. 28:4968. PubMed
  5. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  6. Chang L, et al. 2023. Life Sci Alliance. 6:. PubMed
  7. Nisa BU, et al. 2023. Life (Basel). 13:. PubMed
  8. Seree-Aphinan C, et al. 2023. Front Immunol. 14:1138765. PubMed
  9. Molpeceres C, et al. 2023. Int J Bioprint. 9:720. PubMed
  10. Wu L, et al. 2023. Cell Res. . PubMed
  11. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  12. Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
  13. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  14. Sluzalska K,et al. 2017. Sci Rep.. 10.1038/s41598-017-14004-9. PubMed
  15. Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed
  16. Zhuang J, et al. 2020. Nucleic Acids Res. 48:8870. PubMed
  17. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  18. Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed
  19. Li Y, et al. 2021. Aging (Albany NY). 13:25886. PubMed
  20. Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed
  21. Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
  22. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  23. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  24. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  25. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  26. Yu LC, et al. 2020. Front Neurol. 1.104166667. PubMed
  27. Quintanal-Villalonga A, et al. 2022. STAR Protoc. 3:101776. PubMed
  28. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  29. Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
  30. Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
  31. Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
  32. Chioh FW, et al. 2021. eLife. 10:00. PubMed
  33. Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed
  34. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  35. Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
  36. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  37. Boya M, et al. 2022. Nat Commun. 13:3385. PubMed
  38. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
RRID
AB_2566369 (BioLegend Cat. No. 368509)
AB_2566369 (BioLegend Cat. No. 368510)

Antigen Details

Structure
Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution

Hematopoietic cells, except circulating erythrocytes or platelets.

Function
Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD45
Specificity Alt (DOES NOT SHOW ON TDS):
CD45
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD45 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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