Loading...

Purified anti-human CD146 Antibody

Pricing & Availability
Clone
P1H12 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Muc-18, MCAM, Mel-CAM, S-endo
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
P1H12_Purified_CD146_Antibody_FC_111813
Human cervical cancer cell line HeLa was stained with purified anti-human CD146 (clone P1H12, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • P1H12_Purified_CD146_Antibody_FC_111813
    Human cervical cancer cell line HeLa was stained with purified anti-human CD146 (clone P1H12, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Compare all formats
Cat # Size Price Quantity Check Availability Save
361001 25 µg 57€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
361002 100 µg 101€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD146 is a 118 kD integral transmembrane glycoprotein that is also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Dog, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human umbilical cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: Western blotting3 and IHC1,5. 

Clone P1H12 cross-reacts to Dog2, 5.

Application References
  1. Solovey A, et al. 1997. N. Engl. J. Med. 337:1584. (FC, IHC)
  2. Lamerato-Kozicki AR, et al. 2006. Exp. Hematol. 34:870. (FC)
  3. Balint K, et al. 2005. J. Clin. Invest. 115:3166. (WB)
  4. Neskey DM, et al. 2008. J. Exp. Clin. Cancer Res. 27:61. (ELISA)
  5. Kamstock D, et al. 2006. Cancer Gene Therap. 13:306. (IHC)
Product Citations
  1. Klinker MW, et al. 2017. Proc Natl Acad Sci U S A. E2598:114. PubMed
  2. Pars S, et al. 2021. Cells. 10:. PubMed
  3. Stalin J, et al. 2016. Oncogene. 10.1038/onc.2016.83. PubMed
RRID
AB_2562882 (BioLegend Cat. No. 361001)
AB_2562882 (BioLegend Cat. No. 361002)

Antigen Details

Structure
118 kD integral glycoprotein, Ig superfamily
Distribution

Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells

Function
Adhesion, monocytes transendothelial migration
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Pickl WF, et al. 1997. J. Immunol. 158:2107.
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219.
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.

Gene ID
4162 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD146
Specificity Alt (DOES NOT SHOW ON TDS):
CD146
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F,WB
UniProt
View information about CD146 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account