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Purified anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_Pure_070808.jpg
C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_070808.jpg
    C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_Ly6c_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified Ly6C clone HK1.4 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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128001 50 µg 52€
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128002 500 µg 188€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
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  20. Karhadkar TR, et al. 2021. J Pharmacol Exp Ther. 376:106. PubMed
  21. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  22. Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
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  24. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
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  56. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  57. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  58. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  59. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  60. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  61. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
  62. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  63. Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
  64. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  65. Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
  66. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  67. Zhang C, et al. 2020. Hear Res. 393:107996. PubMed
  68. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
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  72. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  73. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  74. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  75. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
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RRID
AB_1134213 (BioLegend Cat. No. 128001)
AB_1134213 (BioLegend Cat. No. 128002)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6C
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6C
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 10/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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