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TruStain FcX™ (anti-mouse CD16/32) Antibody

Pricing & Availability
Clone
93 (See other available formats)
Regulatory Status
RUO
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
649 publications
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Cat # Size Price Quantity Check Availability Save
101319 50 µg 44€
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101320 500 µg 184€
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Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.

Application References
  1. Oliver AM, et al. 1999. Hybridoma 18:113.
  2. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
  3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  4. Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
  5. Flores M, et al. 2008. FASEB J. 22:3661. PubMed
  6. Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
  7. Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
  8. Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
  9. Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
  10. Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  11. Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
  12. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
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Product Citations
  1. Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed
  2. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  3. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  4. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  5. Haupt F, et al. 2019. Sci Rep. 9:9798. PubMed
  6. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  7. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  8. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  9. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  10. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  11. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  12. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
See More
RRID
AB_1574973 (BioLegend Cat. No. 101319)
AB_1574973 (BioLegend Cat. No. 101320)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD16/32
Specificity Alt (DOES NOT SHOW ON TDS):
CD16/32
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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