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Alexa Fluor® 488 anti-NRF2 Antibody

Pricing & Availability
Clone
W19086B (See other available formats)
Regulatory Status
RUO
Other Names
Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19086B_A-488_NRF2_ICFC_102924
HeLa cells were untreated (negative control, open histogram), or treated with MG-132 (positive control, filled histogram) to induce accumulation of NRF2 by inactivation of the proteosome. The cells were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 488 anti-NRF2 (clone W19086B) or Alexa Fluor® 488 rat IgG2a, κ Isotype Control (Cat. No. 400525) (open histogram, dashed line) (representative histogram for either untreated or treated cells).
  • W19086B_A-488_NRF2_ICFC_102924
    HeLa cells were untreated (negative control, open histogram), or treated with MG-132 (positive control, filled histogram) to induce accumulation of NRF2 by inactivation of the proteosome. The cells were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 488 anti-NRF2 (clone W19086B) or Alexa Fluor® 488 rat IgG2a, κ Isotype Control (Cat. No. 400525) (open histogram, dashed line) (representative histogram for either untreated or treated cells).
Compare all formats See Alexa Fluor® 488 spectral data
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939207 25 tests 128€
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939208 100 tests 316€
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Description

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human NRF2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining.

Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate.

Antigen Details

Structure
NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Transcription factor/Redox balance
Biology Area
Cell Biology, Mitochondrial Function, Transcription Factors
Antigen References
  1. M. da Costa R, et al. 2019. Front In Pharm. 10:3389.
  2. Shoemaker A. 2017. Sci Trans Med. 9:420.
  3. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155.
  4. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.  
Gene ID
4780 View all products for this Gene ID
UniProt
View information about NRF2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All NRF2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-NRF2 W19086B WB,ICC,IP,IHC-P,ICFC
PE anti-NRF2 W19086B ICFC
Alexa Fluor® 647 anti-NRF2 W19086B ICFC
Alexa Fluor® 488 anti-NRF2 W19086B ICFC
Go To Top Version: 1    Revision Date: 10/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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